Human CellExp™ IL-10, Human Recombinant

Catalog number
6466-10
Name
Human CellExp™ IL-10, Human Recombinant
Size
10 µg
Price
344.00 EUR
Supplier

Details

Background
IL10 is produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract.
Concentration
N/A
Molecular weight
17 kDa, non-disulfide bonded homodimer, glycosylated
Synonym
Human Cellexp Human Recombinant IL-10
Other name
B-TCGF, CSIF, TGIF, IL-10, IL10A, MGC126450, MGC126451, Cytokine synthesis inhibitory factor.
NCBI gene number
3586
NCBI gene
IL10
Gene source
Human
NCBI number
P22301
Recombinant
Yes
Source
Human 293 cell expressed
Purification
> 95%
Tested applications
SDS-PAGE, N/A
Level of endotoxin
<1.0 EU per 1 μg of protein
Tested activity
N/A
Biological activity
ED₅₀ is typically 0.3 to 1.5 ng/mL. The specific activity was determined by the dose-dependent simulation of the proliferation of MC/9 cells (mouse mast cell line) in the presence of 200 pg/mL IL-4.
Reesults
0.3 to 1.5 ng/ml
Binding ability
N/A
Unit
N/A
Storage condition
-80°C
Shipping under
gel pack
Physical appearance
Lyophilized
Physical properties
Lyophilized from a PBS solution.
How to reconstitute
Reconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants