Human CellExp™ IL-17A, Human Recombinant

Catalog number
6468-10
Name
Human CellExp™ IL-17A, Human Recombinant
Size
10 µg
Price
197.00 EUR
Supplier

Details

Background
Interleukin-17 is a proinflammatory cytokine produced by activated T cells. IL-17 regulates the activities of NF-kappa B and mitogen-activated protein kinases. IL17 can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of IL-17 are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. The original described IL-17 protein, now known as IL-17A, was identified from a CD4+ T cells DNA library. IL-17 can be induced from primary peripheral blood CD4+ T cells upon stimulation. Supernatant from cells transfected with IL-17 induced IL-16 and IL-18 production and enhanced the surface expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts.
Concentration
N/A
Molecular weight
30 to 35 kDa, homodimer, glycosylated
Synonym
Human Cellexp Human Recombinant IL-17A
Other name
CTLA-8, IL-17, IL-17A, Cytotoxic T-lymphocyte-associated antigen 8.
NCBI gene number
3605
NCBI gene
IL17A
Gene source
Human
NCBI number
Q16552
Recombinant
Yes
Source
Human 293 cell expressed
Purification
> 95%
Tested applications
SDS-PAGE, N/A
Level of endotoxin
<1.0 EU per 1 μg of protein
Tested activity
N/A
Biological activity
ED₅₀ is typically 5 to 25 ng/mL. The specific activity was determined by the dose-dependent induction of IL-6 secretion from NDHF Adult fibroblasts.
Reesults
5 to 25 ng/ml
Binding ability
N/A
Unit
N/A
Storage condition
-80°C
Shipping under
gel pack
Physical appearance
Lyophilized
Physical properties
Lyophilized in 10 mM Tris, pH 7.4 and 250 mM NaCl.
How to reconstitute
Reconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants