Human CellExp™ PDGF-AA, Human Recombinant

Catalog number
6476-50
Name
Human CellExp™ PDGF-AA, Human Recombinant
Size
50 µg
Price
1333.00 EUR
Supplier

Details

Background
PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGF is stored in platelet alpha-granules and released upon platelet activation. The PDGF is involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGF have been identified and named PDGFR-alpha and PDGFR-beta. PDGFR-alpha is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-beta interacts with only PDGF-BB and PDGF-AB.
Concentration
N/A
Molecular weight
35, 40 and 45 kDa, homodimer, glycosylated
Synonym
Human Cellexp Human Recombinant PDGF-AA
Other name
Glioma-derived growth factor, GDGF, Osteosarcoma-derived Growth Factor, ODGF, PDGF-AA, PDGF-1.
NCBI gene number
5154
NCBI gene
PDGFA
Gene source
Human
NCBI number
P04085
Recombinant
Yes
Source
Human 293 cell expressed
Purification
> 95%
Tested applications
SDS-PAGE, N/A
Level of endotoxin
<1.0 EU per 1 μg of protein
Tested activity
N/A
Biological activity
ED₅₀ is typically 200 to 1000 ng/mL. The specific activity was determined by the dose-dependent stimulation of the proliferation of 3T3 cells.
Reesults
200 to 1000 ng/ml
Binding ability
N/A
Unit
N/A
Storage condition
-80°C
Shipping under
gel pack
Physical appearance
Lyophilized
Physical properties
Lyophilized from a solution of 10 mM AcOH.
How to reconstitute
Reconstitute in sterile PBS containing 10 mM AcOH.
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants