Human CellExp™ VEGF 165, Human Recombinant

Catalog number
6485-50
Name
Human CellExp™ VEGF 165, Human Recombinant
Size
50 µg
Price
1154.00 EUR
Supplier

Details

Background
VEGF is an important signaling protein involved in both vasculogenesis and angiogenesis. As its name implies, VEGF activity has been mostly studied on cells of the vascular endothelium, although it does have effects on a number of other cell types (e.g. stimulation monocyte/ macrophage migration, neurons, cancer cells, kidney epithelial cells).VEGF mediates increased vascular permeability, induces angiogenesis, vasculogenesis and endothelial cell growth, promotes cell migration, and inhibits apoptosis. In vitro, VEGF has been shown to stimulate endothelial cell mitogenesis and cell migration. VEGF is also a vasodilator and increases microvascular permeability and was originally referred to as vascular permeability factor. Elevated levels of this protein are linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy.
Concentration
N/A
Molecular weight
45 kDa, homodimer, glycosylated
Synonym
Human Cellexp Human Recombinant VEGF 165
Other name
Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, Folliculostellate cell-derived growth factor, Glioma-derived endothelial cell mitogen, VEGF, MGC70609.
NCBI gene number
7422
NCBI gene
VEGFA
Gene source
Human
NCBI number
P15692
Recombinant
Yes
Source
Human 293 cell expressed
Purification
> 95%
Tested applications
SDS-PAGE, N/A
Level of endotoxin
<1.0 EU per 1 μg of protein
Tested activity
N/A
Biological activity
ED₅₀ is typically 2 to 10 ng/mL. The specific activity was determined by the dose-dependent stimulation of the proliferation of HUVEC cells (Human Umbilical Vein Endothelial Cells).
Reesults
2 to 10 ng/ml
Binding ability
N/A
Unit
N/A
Storage condition
-80°C
Shipping under
gel pack
Physical appearance
Lyophilized
Physical properties
Lyophilized in PBS.
How to reconstitute
Reconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants