IL-2 Receptor α, Human Recombinant

Catalog number
7100-10
Name
IL-2 Receptor α, Human Recombinant
Size
10 μg
Price
285.00 EUR
Supplier

Details

Synonyms
soluble IL-2 receptor, TAC-antigen, CD25 antigen
Alternates names
soluble IL-2 receptor, TAC-antigen, CD25 antigen
Taglines
A receptor present on immune cells like lymphocytes, which binds and responds to IL-2 cytokine
Biomolecule/Target
IL-2 Rα
NCBI Gene ID #.
3561
NCBI Gene Symbol
IL2RA
Gene Source
Human
Accession #
P31785
Recombinant
Yes
Source
(BTI-Tn-5B1-4) Hi-5 Insect Cells
Purity
N/A
Endotoxin Level
<0.1 ng/μg of protein (
Activity (Specifications/test method)
Determined by its ability to increase the proliferation effect of IL-2 in mouse CTLL-2 cells. In the presence of 1 ng/ml of recombinant IL-2, the expected ED50 for this effect is between 0.5-1.5 μg/ml.
Biological activity
Determined by its ability to increase the proliferation effect of IL-2 in mouse CTLL-2 cells. In the presence of 1 ng/ml of recombinant IL-2, the expected ED50 for this effect is between 0.5-1.5 μg/ml.
Results
N/A
Molecular Weight
24.8 kDa (31 kDa on SDSPAGE due to glycosylation)
Storage Temp.
-20°C
Shipping
Gel Pack
Shelf Life
1 year
Concentration
N/A
Appearance
Lyophilized powder
Physical form description
Sterile filtered through a 0.2 micron filter. Lyophilized from 1x PBS
Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in 1x PBS pH 7.2 to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Handling
Centrifuge the vial prior to opening.
Usage
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Description
The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
Additional source
Recombinants or rec. proteins
Group
recombinants