SPARC/Osteonectin, human recombinant

Catalog number
7204-10
Name
SPARC/Osteonectin, human recombinant
Size
10 μg
Price
235.00 EUR
Supplier

Details

Synonyms
Secreted protein acidic and rich in cysteine, BM-40, ON
Alternates names
Secreted protein acidic and rich in cysteine, BM-40, ON
Taglines
Involved in a variety of cellular activities
Biomolecule/Target
Osteonectin
NCBI Gene ID #.
6678
NCBI Gene Symbol
SPARC
Gene Source
Human
Accession #
P09486
Recombinant
Yes
Source
CHO cells
Purity
N/A
Endotoxin Level
< 0.1 ng/μg of protein (
Activity (Specifications/test method)
Determined by its ability to increase alkaline phosphatase activity in differentiating MC3T3 cells using a concentration of 0.5 – 0.7 µg/ml
Biological activity
Determined by its ability to increase alkaline phosphatase activity in differentiating MC3T3 cells using a concentration of 0.5 – 0.7 µg/ml
Results
N/A
Molecular Weight
43.7 kDa
Storage Temp.
-20°C
Shipping
Gel Pack
Shelf Life
1 year
Concentration
N/A
Appearance
Lyophilized powder
Physical form description
Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium Phosphate, pH 7.6
Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Handling
Centrifuge the vial prior to opening.
Usage
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants