Puromycin Dihydrochloride for cell activation
Catalog number
1860-500
Name
Puromycin Dihydrochloride for cell activation
Size
500 mg
Price
806.00 EUR
Supplier
Details
Description
A nucleoside antibiotic. It inhibits protein synthesis by disrupting peptide transfer on ribosomes causing premature chain termination during translation. It is a potent translational inhibitor in both prokaryotic and eukaryotic cells. Resistance to puromycin is conferred by the puromycin N-acetyl-transferase gene (pac) from Streptomyces. Puromycin has a fast mode of action, causing rapid cell death at low antibiotic concentrations. Adherent mammalian cells are sensitive to concentrations of 2 to 5 µg/ml, while cells in suspension are sensitive to concentrations as low as 0.5 to 2 µg/ml.
Peptide sequence
N/A
CAS number
58-58-2
Molecular weight
544.43
Category
Biochemicals
Other name
3′-[α-Amino-p-methoxyhydrocinnamamido]-3′-deoxy-N,N-dimethyladenosine dihydrochloride
Molecular formula
C₂₂H₂₉N₇O₅ · 2HCl
Physical appearance
White to off-white solid
Supplied with
N/A
Is this a salt?
No
Is it cell-permeable?
No
Purification
≥98% by HPLC
Reconstitute instructions
H₂O (~50 mg/ml)
Storage condition
-20°C
Shipping condition
gel pack
Maximum time for storage
24 months
Storage instructions
Protect from air and moisture
Tissue
cell
Additional description
For cells, cell lines and tissues in culture till half confluency.