Human CellExp™ IL-17E, human recombinant

Catalog number
7224-10
Name
Human CellExp™ IL-17E, human recombinant
Size
10 μg
Price
371.00 EUR
Supplier

Details

Target
IL-17E
Background
Interleukin 25 (IL25) also known as Interleukin-17E (IL-17E), is a distinct member of the IL17 cytokine family comprised of at least six members sharing a conserved cysteine-knot structure but divergent at the N-terminus. IL-25 is secreted by type 2 helper T cells (Th2) and mast cells. IL25 can induce NF-κB activation, and stimulate the production of IL8. Both this cytokine and IL17B are ligands for the cytokine receptor IL17RB. IL-25 induces the production of other cytokines, including IL-4, IL-5 and IL-13 in multiple tissues, which stimulate the expansion of eosinophils. This cytokine is an important molecule controlling immunity of the gut and has been implicated in chronic inflammation associated with the gastrointestinal tract. IL-25 can kill some types of breast cancer cells.
Concentration
N/A
Molecular weight
This protein is fused with 6 × His tag at the C-terminus, has a calculated MW of 16.7 kDa. The predicted N-terminus is Tyr 33. DTT-reduced Protein migrates as 24 kDa, 27 kDa and 31 kDa due to different glycosylation.
Synonym
IL-17E, IL-25, Interleukin-25
Other name
IL-17E, IL-25, Interleukin-25
NCBI gene number
64806
NCBI gene
IL-17E
Gene source
Human
NCBI number
Q9H293
Recombinant
Yes
Source
HEK 293 cells
Purification
≥95%
Tested applications
SDS-PAGE, HPLC
Level of endotoxin
<0.1 ng/μg of protein (
Tested activity
The activity is determined by the dose-dependent production of IL-8 by human PBMCs. The expected ED50 for this effect is 12-25 ng/ml.
Biological activity
The activity is determined by the dose-dependent production of IL-8 by human PBMCs. The expected ED50 for this effect is 12-25 ng/ml.
Reesults
N/A
Binding ability
N/A
Unit
N/A
Storage condition
-20°C
Shipping under
Gel Pack
Physical appearance
Lyophilized powder
Physical properties
Lyophilized from 0.22 μm filtered solution in PBS. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
How to reconstitute
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants