Human CellExp™ Olfactomedin, human recombinant

Catalog number
7225-10
Name
Human CellExp™ Olfactomedin, human recombinant
Size
10 μg
Price
268.00 EUR
Supplier

Details

Target
Olfactomedin
Background
Olfactomedin-4 (OLM-4) also known as OLFM4, antiapoptotic protein GW112, G-CSF-stimulated clone 1 protein (hGC-1), hOLfD, is a secreted protein which contains one olfactomedin-like domain. OLFM4 is expressed during myeloid lineage development. Much higher expression in bone marrow neutrophils than in peripheral blood neutrophils (at protein level). Strongly expressed in the prostate, small intestine and colon and moderately expressed in the bone marrow and stomach. Overexpressed in some pancreatic cancer tissues. OLFM4 may promote proliferation of pancreatic cancer cells by favoring the transition from the S to G2/M phase. In myeloid leukemic cell lines, inhibits cell growth and induces cell differentiation and apoptosis. OLFM4 may play a role in the inhibition of EIF4EBP1 phosphorylation/deactivation. Facilitates cell adhesion, most probably through interaction with cell surface lectins and cadherin. The human OLFM4 is also thought to be a useful marker for early myeloid development.
Concentration
N/A
Molecular weight
This protein is fused with 6 × His tag at the N-terminus, has a calculated MW of 56 kDa. The predicted N-terminus is Asp 21. DTT-reduced protein migrates as 65-70 kDa due to glycosylation.
Synonym
OLFM4, GC1, GW112, Olfactomedin-4, KIAA4294, OLM4, OlfD, UNQ362, hGC-1, hOLfD
Other name
OLFM4, GC1, GW112, Olfactomedin-4, KIAA4294, OLM4, OlfD, UNQ362, hGC-1, hOLfD
NCBI gene number
283298
NCBI gene
OLFML1
Gene source
Human
NCBI number
Q6UWY5
Recombinant
Yes
Source
HEK 293 cells
Purification
≥95%
Tested applications
SDS-PAGE, HPLC
Level of endotoxin
<0.1 ng/μg of protein (
Tested activity
N/A
Biological activity
N/A
Reesults
N/A
Binding ability
N/A
Unit
N/A
Storage condition
-20°C
Shipping under
Gel Pack
Physical appearance
Lyophilized powder
Physical properties
Lyophilized from 0.22 μm filtered solution in PBS. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
How to reconstitute
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants