Human CellExp™ EGF, human recombinant

EGF

Human epidermal growth factor (EGF) is also known as HOMG4 and URG, and is a growth factor that plays an important role in the regulation of cell growth, proliferation, and differentiation by binding to its receptor EGFR. Epidermal growth factor can be found in human platelets, macrophages, urine, saliva, milk, and plasma. EGF is the founding member of the EGF-family of proteins. Members of this protein family have highly similar structural and functional characteristics. All family members contain one or more repeats of the conserved amino acid sequence. The biological effects of salivary EGF include healing of oral and gastroesophageal ulcers, inhibition of gastric acid secretion, stimulation of DNA synthesis as well as mucosal protection from intraluminal injurious factors such as gastric acid, bile acids, pepsin, and trypsin and to physical, chemical and bacterial agents. Because of the increased risk of cancer by EGF, inhibiting it decreases cancer risk.

N/A

This protein has a calculated MW of 6.3 kDa. DTT-reduced protein migrates as 6.3 kDa estimated polypeptide in SDS-PAGE.

EGF, URG, HOMG4, epidermal growth factor

EGF, URG, HOMG4, epidermal growth factor

1950

EGF

Human

P01133

Yes

HEK 293 cells

≥97%

SDS-PAGE, HPLC

< 0.1 ng/μg of protein (

Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 20-100 pg/mL.

Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 20-100 pg/mL.

N/A

N/A

N/A

-20°C

Gel Pack

Lyophilized powder

Lyophilized from 0.22 μm filtered solution in PBS. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

N/A

Centrifuge the vial prior to opening.

For Research Use Only! Not to be used in humans

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Recombinants or rec. proteins

recombinants