Human CellExp™ VEGF-C, human recombinant

Catalog number
7231-10
Name
Human CellExp™ VEGF-C, human recombinant
Size
10 μg
Price
371.00 EUR
Supplier

Details

Target
VEGF-C
Background
Vascular endothelial growth factor C is also known as VEGFC, Flt4-L and VRP; it contains the C-terminal propeptide which has an unusual structure with tandemly repeated cysteine-rich motifs. Upon biosynthesis, VEGFC is secreted as a non-covalent momodimer in an anti-parallel fashion. VEGFC is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family, is active in angiogenesis, lymphangiogenesis and endothelial cell growth and survival, and can also affect the permeability of blood vessels. This secreted protein undergoes a complex proteolytic maturation, generating multiple processed forms that bind and activate VEGFR-3 receptors. Only the fully processed form can bind and activate VEGFR-2 receptors. The structure and function of this protein is similar to those of vascular endothelial growth factor D (VEGF-D). VEGFC may function in angiogenesis of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Overexpression of VEGF-C causes lymphatics to enlarge possibly facilitates metastasis.
Concentration
N/A
Molecular weight
The protein is fused with 6×His tag at the C-terminus, has a calculated MW of 15 kDa. The predicted N-terminus is Thr 103. DTT-reduced Protein migrates as 18-23 kDa due to glycosylation.
Synonym
VEGFC, Flt4-L, VRP
Other name
VEGFC, Flt4-L, VRP
NCBI gene number
7424
NCBI gene
VEGF-C
Gene source
Human
NCBI number
P49767
Recombinant
Yes
Source
HEK 293 cells
Purification
≥97%
Tested applications
SDS-PAGE, HPLC
Level of endotoxin
<0.1 ng/μg of protein (
Tested activity
Measured in a cell proliferation assay using HMVEC human microvascular endothelial cells. The ED50 for this effect is typically 0.15-0.7 μg/ml.
Biological activity
Measured in a cell proliferation assay using HMVEC human microvascular endothelial cells. The ED50 for this effect is typically 0.15-0.7 μg/ml.
Reesults
N/A
Binding ability
N/A
Unit
N/A
Storage condition
-20°C
Shipping under
Gel Pack
Physical appearance
Lyophilized powder
Physical properties
Lyophilized from 0.22 μm filtered solution in PBS. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
How to reconstitute
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants