Human CellExp™ PLGF/PIGF2/PGF, human recombinant

Catalog number
7238-10
Name
Human CellExp™ PLGF/PIGF2/PGF, human recombinant
Size
10 μg
Price
469.00 EUR
Supplier

Details

Target
PLGF
Background
Placental growth factor (PGF) also known as vascular endothelial growth factor-related protein, PLGF and PlGF2, is a member of the VEGF (vascular endothelial growth factor) sub-family - a key molecule in angiogenesis and vasculogenesis, in particular during embryogenesis. The main source of PGF during pregnancy is the placental trophoblast. PGF is also expressed in many other tissues, including the villous trophoblast. PGF is actived in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. PlGF2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner. Also promotes cell tumor growth.
Concentration
N/A
Molecular weight
The protein is fused with 6×His tag at the C-terminus, has a calculated MW of 20.2 kDa. The predicted N-terminus is Leu 19. DTT-reduced Protein migrates as 28-33 kDa due to glycosylation.
Synonym
PGF, PLGF, PlGF2, PlGF, PGFL, SHGC-10760
Other name
PGF, PLGF, PlGF2, PlGF, PGFL, SHGC-10760
NCBI gene number
5228
NCBI gene
PLGF
Gene source
Human
NCBI number
P49763
Recombinant
Yes
Source
HEK 293 cells
Purification
≥95%
Tested applications
SDS-PAGE, HPLC
Level of endotoxin
<0.1 ng/μg of protein (
Tested activity
Measured in a cell proliferation assay using MDA - MB – 231 human breast cancer cells. The ED50 for this effect is typically 1 - 10 µg/ml.
Biological activity
Measured in a cell proliferation assay using MDA - MB – 231 human breast cancer cells. The ED50 for this effect is typically 1 - 10 µg/ml.
Reesults
N/A
Binding ability
N/A
Unit
N/A
Storage condition
-20°C
Shipping under
Gel Pack
Physical appearance
Lyophilized powder
Physical properties
Lyophilized from 0.22 μm filtered solution in PBS. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
How to reconstitute
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants