Human CellExp™ MMP-1, human recombinant

Catalog number
7244-50
Name
Human CellExp™ MMP-1, human recombinant
Size
50 μg
Price
1051.00 EUR
Supplier

Details

Target
MMP-1
Background
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive pro-proteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. Thrombospondins, intervertebral disc proteins, regulate the effective levels of matrix metalloproteinases (MMPs) 2 and 9, which are key effectors of ECM remodeling. Matrix metalloproteinase-1 (MMP-1) also known as interstitial collagenase and fibroblast collagenase. MMP1 is expressed by fibroblasts, keratinocytes, endothelial cells, monocytes and macrophages. MMP-1 breaks down the interstitial collagens, types I, II, and III. MMP1 can degrade a broad range of substrates including types I, II, III, VII, VIII, and X collagens as well as casein, gelatin, myelin basic protein, L Selectin, proTNF, IL1β, IGFBP3, IGFBP5, pro MMP2 and pro MMP9.
Concentration
N/A
Molecular weight
This protein is fused with 6×His tag at the N-terminus, has a calculated MW of 52.7 kDa. The predicted N-terminus is Phe 20. DTT-reduced Protein migrates as 52-55 kDa.
Synonym
MMP1, CLG, CLGN
Other name
MMP1, CLG, CLGN
NCBI gene number
4312
NCBI gene
MMP-1
Gene source
Human
NCBI number
P03956
Recombinant
Yes
Source
HEK 293 cells
Purification
≥95%
Tested applications
SDS-PAGE, HPLC
Level of endotoxin
<0.1 ng/μg of protein (
Tested activity
Measured by its ability to cleave a fluorogenic peptide substrate Mca - KPLGL - Dpa - AR - NH2. The specific activity is> 200 pmol/min/µg, as measured under the described conditions.
Biological activity
Measured by its ability to cleave a fluorogenic peptide substrate Mca - KPLGL - Dpa - AR - NH2. The specific activity is> 200 pmol/min/µg, as measured under the described conditions.
Reesults
N/A
Binding ability
N/A
Unit
N/A
Storage condition
-20°C
Shipping under
Gel Pack
Physical appearance
Lyophilized powder
Physical properties
Lyophilized from 0.22 μm filtered solution in MES, NaCl, CaCl2 and Brij­35. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
How to reconstitute
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants