Human CellExp™ GFER/HPO/ALR, human recombinant

Catalog number
7269-10
Name
Human CellExp™ GFER/HPO/ALR, human recombinant
Size
10 μg
Price
469.00 EUR
Supplier

Details

Target
GFER
Background
Growth factor, augmenter of liver regeneration (GFER) is also known as FAD-linked sulfhydryl oxidase ALR, which belongs to the Erv1/ALR family of proteins. This family can be found in higher and lower eukaryotes. There are two isoform of GFER: Isoform 1 and isoform 2. Isoform 2 missing 1 – 80 aa. Isoform 1 is mainly located in mitochondrion intermembrane space, while Isoform 2 is secreted to cytoplasm. Isoform 1 of GFER regenerates the redox-active disulfide bonds in CHCHD4/MIA40, a chaperone essential for disulfide bond formation and protein folding in the mitochondrial intermembrane space. The reduced form of CHCHD4/MIA40 forms a transient intermolecular disulfide bridge with GFER/ERV1, resulting in regeneration of the essential disulfide bonds in CHCHD4/MIA40, while GFER/ERV1 becomes re-oxidized by donating electrons to cytochrome c or molecular oxygen. The isoform 2 of GFER may act as an autocrine hepatotrophic growth factor promoting liver regeneration.
Concentration
N/A
Molecular weight
Calculated MW of 15.2 kDa with no tag. The predicted N-terminus is Met 81. DTT-reduced protein migrates as 16.0 kDa.
Synonym
GFER, ALR, ERV1, HERV1, HPO, HPO1, HPO2, HSS, Hepatopoietin
Other name
GFER, ALR, ERV1, HERV1, HPO, HPO1, HPO2, HSS, Hepatopoietin
NCBI gene number
2671
NCBI gene
GFER
Gene source
Human
NCBI number
P55789
Recombinant
Yes
Source
HEK 293 cells
Purification
≥92%
Tested applications
SDS-PAGE, N/A
Level of endotoxin
<0.1 ng/μg of protein (
Tested activity
N/A
Biological activity
N/A
Reesults
N/A
Binding ability
N/A
Unit
N/A
Storage condition
-20°C
Shipping under
Gel Pack
Physical appearance
Lyophilized powder
Physical properties
Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 100 mM NaCl, pH 8.0. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
How to reconstitute
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 100 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants