Human CellExp™ IL-1 Alpha, human recombinant

Catalog number
7275-10
Name
Human CellExp™ IL-1 Alpha, human recombinant
Size
10 μg
Price
371.00 EUR
Supplier

Details

Target
IL-1a
Background
Interleukin-1 alpha (IL1a) is also known as IL-1A, IL1, IL1-ALPHA, IL1F1, and is a cytokine of the interleukin-1 family. IL1a possesses a wide spectrum of metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. It binds to the interleukin-1 receptor. IL-1α is constitutively produced by epithelial cells. It is found in substantial amounts in normal human epidermis and is distributed in a 1:1 ratio between living epidermal cells and stratum corneum. The constitutive production of large amounts of IL-1α precursor by healthy epidermal keratinocytes interfere with the important role of IL-1α in immune responses, assuming skin as a barrier, which prevents the entry of pathogenic microorganisms into the body. In vitro, IL-1α possesses biological effect on cells in the picomolar to femtomolar range. In vivo, shortly after an onset of an infection into organism, IL-1α activates a set of immune system response processes.
Concentration
N/A
Molecular weight
Calculated MW of 18.0 kDa with no tag. DTT-reduced protein migrates as 22 kDa. in SDS-PAGE
Synonym
IL1A, IL-1A, IL1, IL1-ALPHA, IL1ALPHA, IL1F1, IL-1α, IL1α, Interleukin-1 alpha, Interleukin-1, Interleukin
Other name
IL1A, IL-1A, IL1, IL1-ALPHA, IL1ALPHA, IL1F1, IL-1α, IL1α, Interleukin-1 alpha, Interleukin-1, Interleukin
NCBI gene number
3552
NCBI gene
IL-1a
Gene source
Human
NCBI number
P01583
Recombinant
Yes
Source
HEK 293 cells
Purification
≥97%
Tested applications
SDS-PAGE, N/A
Level of endotoxin
<0.1 ng/μg of protein (
Tested activity
The bio-activity was determined by dose-dependent stimulation of the proliferation of mouse D10S cells. The ED50 1X10^9 Unit/mg.
Biological activity
The bio-activity was determined by dose-dependent stimulation of the proliferation of mouse D10S cells. The ED50 1X10^9 Unit/mg.
Reesults
N/A
Binding ability
N/A
Unit
N/A
Storage condition
-20°C
Shipping under
Gel Pack
Physical appearance
Lyophilized powder
Physical properties
Lyophilized from 0.22 μm filtered solution in in PBS, pH 7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
How to reconstitute
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Description
The CellExp™ IL-1 Alpha, recombinant is a α- or alpha protein sometimes glycoprotein present in blood.
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Gene
The Interleukin-1 family (IL-1 family) is a group of 11 cytokines, which plays a central role in the regulation of immune and inflammatory responses to infections or sterile insults. Rec. E. coli interleukin-1 for cell culture or antibody production.
Additional source
Recombinants or rec. proteins
Group
recombinants