TNFSF8, human recombinant

Catalog number
7331-100
Name
TNFSF8, human recombinant
Size
50 μg
Price
349.00 EUR
Supplier

Details

Target
TNFSF8
Background
TNFSF8 is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF8/CD30, which is a cell surface antigen and a marker for Hodgkin lymphoma and related hematologic malignancies. The engagement of this cytokine expressed on B cell surface plays an inhibitory role in modulating Ig class switch. TNFSF8 was shown to enhance cell proliferation of some lymphoma cell lines, while to induce cell death and reduce cell proliferation of other lymphoma cell lines. The pleiotropic biologic activities of this cytokine on different CD30+ lymphoma cell lines may play a pathophysiologic role in Hodgkin's and some non-Hodgkin's lymphomas. Recombinant human TNFSF8 protein, fused to His-tag at N-terminus, was expressed in E.coli.
Concentration
0.5 mg/ml
Molecular weight
22 kDa (195 aa, 63-234 aa + His Tag), confirmed by MALDI-TOF.
Synonym
tumor necrosis factor ligand superfamily member 8, CD153, CD30L, CD30LG
Other name
tumor necrosis factor ligand superfamily member 8, CD153, CD30L, CD30LG
NCBI gene number
944
NCBI gene
TNFSF8
Gene source
Human
NCBI number
P32971
Recombinant
Yes
Source
E. coli
Purification
≥80%
Tested applications
SDS-PAGE, N/A
Level of endotoxin
<1.0 EU per 1 microgram of protein
Tested activity
N/A
Biological activity
N/A
Reesults
N/A
Binding ability
N/A
Unit
N/A
Storage condition
-80°C
Shipping under
Dry Ice
Physical appearance
Liquid
Physical properties
0.5 mg/ml in 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol and 0.4 M Urea.
How to reconstitute
N/A
Aa sequence
MGSSHHHHHH SSGLVPRGSH MGSQRTDSIP NSPDNVPLKG GNCSEDLLCI LKRAPFKKSW AYLQVAKHLN KTKLSWNKDG ILHGVRYQDG NLVIQFPGLY FIICQLQFLV QCPNNSVDLK LELLINKHIK KQALVTVCES GMQTKHVYQN LSQFLLDYLQ VNTTISVNVD TFQYIDTSTF PLENVLSIFL YSNSD
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants