Human CellExp™ CD244 / 2B4 / SLAMF4, human recombinant

Catalog number
7374-50
Name
Human CellExp™ CD244 / 2B4 / SLAMF4, human recombinant
Size
50 μg
Price
697.00 EUR
Supplier

Details

Target
CD244/2B4/SLAMF4
Background
Natural killer cell receptor 2B4 is also known as NK cell type I receptor protein 2B4 (NKR2B4 or h2B4), SLAM family member 4 (SLAMF4), Signaling lymphocytic activation molecule 4, CD antigen CD244. NKR2B4 / CD244 contain two Ig-like (immunoglobulin-like) domains. CD244 is expressed in spleen, PBL, followed by lung, liver, testis and small intestine. CD244 interacts with CD48. Following phosphorylation, CD244 is able to recruit PTPN11/SHP-2 and SH2D1A/SAP. SLAMF4 modulate other receptor-ligand interactions to enhance leukocyte activation. CD244/2B4 is the only heterophilic receptor of SLAM family.
Concentration
N/A
Molecular weight
This protein is fused with polyhistidine tag at the C-terminus, and has a calculated MW of 23.1 kDa. The predicted N-terminus is Cys 22. DTT-reduced Protein migrates as 40-50 kDa due to glycosylation.
Synonym
CD244, 2B4, SLAMF4, NKR2B4, NAIL, h2B4
Other name
CD244, 2B4, SLAMF4, NKR2B4, NAIL, h2B4
NCBI gene number
51744
NCBI gene
CD244
Gene source
Human
NCBI number
Q9BZW8
Recombinant
Yes
Source
HEK293 cells
Purification
≥95%
Tested applications
SDS-PAGE, N/A
Tested activity
N/A
Biological activity
Measured by its binding ability in a functional ELISA. Immobilized rh CD244 / 2B4 at 2 μg/ml (100 μl/well) can bind human CD48. The EC50 of human CD48 is 0.39 μg/ml.
Reesults
Measured by its binding ability in a functional ELISA. Immobilized rh CD244 / 2B4 at 2 μg/ml (100 μl/well) can bind human CD48. The EC50 of human CD48 is 0.39 μg/ml.
Binding ability
N/A
Unit
N/A
Storage condition
-20°C
Shipping under
Gel Pack
Physical appearance
Lyophilized
Physical properties
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
How to reconstitute
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants