Human CellExp™ TNFR1 / CD120a / TNFRSF1A, human recombinant

Catalog number
7382-10
Name
Human CellExp™ TNFR1 / CD120a / TNFRSF1A, human recombinant
Size
10 μg
Price
246.00 EUR
Supplier

Details

Target
TNFR1 / CD120a / TNFRSF1A
Background
Tumor necrosis factor receptor 1 (TNF-R1) also known as Tumor necrosis factor receptor superfamily member 1A (TNFRSF1A), TNFAR, CD antigen CD120a, belongs to the tumor necrosis factor receptor superfamily. TNF-R1 contains one death domain and four TNFR-Cys repeats. TNF-R1 is the receptor of TNFSF2 / TNF-alpha and homotrimeric TNFSF1 / lymphotoxin - alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. TNF-R1 contributes to the induction of non - cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase. Defects in TNFRSF1A are the cause of familial Hibernian fever (FHF).
Concentration
N/A
Molecular weight
This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 22 kDa. The predicted N-terminus is Ile 22. DTT-reduced Protein migrates as 30-35 kDa due to different glycosylation.
Synonym
TNFR1, CD120a, TNFRSF1A, FPF, TBP1, TNF-R, TNF-R-I, TNF-R55, TNFAR, TNFR55, TNFR60, p55, p60.
Other name
TNFR1, CD120a, TNFRSF1A, FPF, TBP1, TNF-R, TNF-R-I, TNF-R55, TNFAR, TNFR55, TNFR60, p55, p60.
NCBI gene number
7132
NCBI gene
TNFRSF1A
Gene source
Human
NCBI number
P19438
Recombinant
Yes
Source
HEK293 cells
Purification
≥92%
Tested applications
SDS-PAGE, N/A
Tested activity
N/A
Biological activity
Measured by its binding ability in a functional ELISA. Immobilized human TNFα at 10 μg/mL (100 μl/well) can bind biotinylated human TNFRSF1A. The EC50 of biotinylated human TNFRSF1A is 0.02 μg/mL.
Reesults
Measured by its binding ability in a functional ELISA. Immobilized human TNFα at 10 μg/mL (100 μl/well) can bind biotinylated human TNFRSF1A. The EC50 of biotinylated human TNFRSF1A is 0.02 μg/mL.
Binding ability
N/A
Unit
N/A
Storage condition
-20°C
Shipping under
Gel Pack
Physical appearance
Lyophilized
Physical properties
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
How to reconstitute
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants