Human CellExp™ BACE1, human recombinant (Native)

Catalog number
7398-10
Name
Human CellExp™ BACE1, human recombinant (Native)
Size
10 μg
Price
300.00 EUR
Supplier

Details

Target
BACE1
Background
Beta-secretase 1 (BACE1), also known as beta-site APP cleaving enzyme 1 (beta-site amyloid precursor protein cleaving enzyme 1), memapsin-2 (membrane-associated aspartic protease 2), and aspartyl protease 2 (ASP2), β-Secretase , and is a member of the peptidase A1 protein family, BACE1 is a type I integral membrane glycoprotein and aspartic protease that is found mainly in the Golgi. BACE1 is an aspartic-acid protease important in the pathogenesis of Alzheimer's disease, and in the formation of myelin sheaths in peripheral nerve cells.[1] The transmembrane protein contains two active site aspartate residues in its extracellular protein domain and may function as a dimer. This protease is responsible for the proteolytic processing of the amyloid precursor protein (APP). Generation of the 40 or 42 amino acid-long amyloid-β peptides that aggregate in the brain of Alzheimer's patients requires two sequential cleavages of the APP. Extracellular cleavage of APP by BACE creates a soluble extracellular fragment and a cell membrane-bound fragment referred to as C99. The elevation of BACE1 levels can be induced by amyloid plaques surrounding neurons at early stages of pathology before neuron death occurs, and may drive a positive-feedback loop in AD.
Concentration
N/A
Molecular weight
This protein contains no “tags” and has a calculated MW of 49 kDa. The predicted N-terminus is Thr22. DTT-reduced protein migrates as 50-65 kDa polypeptide in SDS-PAGE due to glycosylation.
Synonym
BACE1, ASP2, BACE, FLJ90568, HSPC104, KIAA1149, Beta-secretase-1, memapsin-2, aspartyl-protease-2, beta-site-APP-cleaving-enzyme-1
Other name
BACE1, ASP2, BACE, FLJ90568, HSPC104, KIAA1149, Beta-secretase-1, memapsin-2, aspartyl-protease-2, beta-site-APP-cleaving-enzyme-1
NCBI gene number
23621
NCBI gene
BACE1
Gene source
Human
NCBI number
P56817
Recombinant
Yes
Source
HEK293 cells
Purification
≥98%
Tested applications
SDS-PAGE, N/A
Tested activity
N/A
Biological activity
Measured by its ability to cleave a fluorescent peptide substrate Mca-Ser-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Arg-Lys(Dnp)-Arg-Arg-NH2. Cleavage of the substrate can be measured using excitation and emission wavelengths of 320 and 405 nm, respectively. The specific activity is> 7.5 pmoles /min /μg.
Reesults
Measured by its ability to cleave a fluorescent peptide substrate Mca-Ser-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Arg-Lys(Dnp)-Arg-Arg-NH2. Cleavage of the substrate can be measured using excitation and emission wavelengths of 320 and 405 nm, respectively. The specific activity is> 7.5 pmoles /min /μg.
Binding ability
N/A
Unit
N/A
Storage condition
-20°C
Shipping under
Gel Pack
Physical appearance
Lyophilized
Physical properties
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
How to reconstitute
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants