Human CellExp™ ErbB1/EGFR, mouse recombinant

Catalog number
7402-10
Name
Human CellExp™ ErbB1/EGFR, mouse recombinant
Size
10 μg
Price
300.00 EUR
Supplier

Details

Target
ErbB1
Background
The epidermal growth factor receptor (EGFR; ErbB-1; HER1) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer.
Concentration
N/A
Molecular weight
This protein has a C-terminal 6×His tag and has a calculated MW of 70 kDa. The predicted N-terminus is Leu 25. In DTT-reduced SDS-PAGE, protein migrates as 110-115 kDa.
Synonym
EGFR, ERBB, ERBB1, HER1, PIG61, mENA
Other name
EGFR, ERBB, ERBB1, HER1, PIG61, mENA
NCBI gene number
13649
NCBI gene
EGFR
Gene source
Mouse
NCBI number
Q01279
Recombinant
Yes
Source
HEK293 cells
Purification
≥95%
Tested applications
SDS-PAGE, N/A
Tested activity
N/A
Biological activity
Measured by its binding ability in a functional ELISA. Immobilized mouse EGF at 10 μg/ml can bind mouse EGFR with a linear range of 2.5–1000 ng/ml.
Reesults
Measured by its binding ability in a functional ELISA. Immobilized mouse EGF at 10 μg/ml can bind mouse EGFR with a linear range of 2.5–1000 ng/ml.
Binding ability
N/A
Unit
N/A
Storage condition
-20°C
Shipping under
Gel Pack
Physical appearance
Lyophilized
Physical properties
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
How to reconstitute
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin name
Mus musculus
Additional source
Recombinants or rec. proteins
Group
recombinants