Human CellExp™ ApoA-1, human recombinant

Catalog number
7403-10
Name
Human CellExp™ ApoA-1, human recombinant
Size
10 μg
Price
289.00 EUR
Supplier

Details

Target
ApoA1
Background
ApoA1 is also known as apolipoprotein A-I, ApoA-I , and is the major protein component of high density lipoprotein (HDL) in plasma. It has a specific role in lipid metabolism. Chylomicrons secreted from the intestinal enterocyte also contain ApoA1 but it is quickly transferred to HDL in the bloodstream. The protein promotes cholesterol efflux from tissues to the liver for excretion. It is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. ApoA-I was also isolated as a prostacyclin (PGI2) stabilizing factor, and thus may have an anticlotting effect. Defects in the gene encoding it are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. In addition, it has been shown that ApoA1 is implicated in the anti-endotoxin function of HDL via interaction with lipopolysaccharide or endotoxin.
Concentration
N/A
Molecular weight
This protein is fused with polyhistidine tag at the C-terminus, and has a calculated MW of 29 kDa. The predicted N-terminus is Asp 25. DTT-reduced Protein migrates as 26-30 kDa in SDS-PAGE.
Synonym
Apolipoprotein A-I, APOA1, MGC117399
Other name
Apolipoprotein A-I, APOA1, MGC117399
NCBI gene number
335
NCBI gene
ApoA1
Gene source
Human
NCBI number
P02647
Recombinant
Yes
Source
HEK293 cells
Purification
≥95%
Tested applications
SDS-PAGE, N/A
Tested activity
N/A
Biological activity
Immobilized Human ApoAI at 10 μg/mL (100 μl/well) can bind biotinylated human SCARB1. The EC50 of biotinylated human SCARB1 is 10-100 ng/mL.
Reesults
Immobilized Human ApoAI at 10 μg/mL (100 μl/well) can bind biotinylated human SCARB1. The EC50 of biotinylated human SCARB1 is 10-100 ng/mL.
Binding ability
N/A
Unit
N/A
Storage condition
-20°C
Shipping under
Gel Pack
Physical appearance
Lyophilized
Physical properties
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
How to reconstitute
Centrifuge the vial prior to opening. Reconstitute in sterile deionized water to a concentration of 50 µg/ml. Solubilize for 30 to 60 min. at RT with occasional gentle mixing. Do not vortex. Carrier protein (0.1% HAS or BSA) is strongly recommended for further dilution and long term storage.
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants