Human CellExp™ Cathepsin B, human recombinant

Cathepsin B

Cathepsin B (CTSB) also known as APP secretase (APPS) and CPSB, is an enzymatic protein belonging to the peptidase C1 family. Cathepsin B / CTSB is synthesized as a preproenzyme. Following removal of the signal peptide, the inactive proenzyme undergoes further modifications including removal of the pro region to result in the active enzyme. The catalytic activity of Cathepsin B / APPS contains: Hydrolysis of proteins with broad specificity for peptide bonds; preferentially cleaves -Arg-Arg-|-Xaa bonds in small molecule substrates (thus differing from cathepsin L); In addition to being an endopeptidase, shows peptidyl-dipeptidase activity, liberating C-terminal dipeptides. As a thiol protease, cathepsin B / CPSB is believed to participate in intracellular degradation and turnover of proteins and has also been implicated in tumor invasion and metastasis. Overexpression of cathepsin B has been associated with esophageal adenocarcinoma and other tumors.

N/A

This protein is fused with a 6×His tag at the C-terminus, has a calculated MW of 36.7 kDa (Pro) and 29 kDa (Mature). The predicted N-terminus is Arg18 (pro) or Phe74 (mature). DTT-reduced Protein migrates as 43 kDa and 34 kDa due to glycosylation.

CTSB, CPSB, APPS

CTSB, CPSB, APPS

1508

CTSB

Human

P07858

Yes

HEK293 cells

≥95%

SDS-PAGE, N/A

N/A

Measured by its ability to cleave the fluorogenic peptide substrate Z-LR-AMC. Measured in 100 μl reaction mixture containing 25 mM MES, pH 5.0, 0.01 µg rhCathepsin B, and 10 µM reaction substrate. The specific activity is >2800 pmol/min/µg.

Measured by its ability to cleave the fluorogenic peptide substrate Z-LR-AMC. Measured in 100 μl reaction mixture containing 25 mM MES, pH 5.0, 0.01 µg rhCathepsin B, and 10 µM reaction substrate. The specific activity is >2800 pmol/min/µg.

N/A

N/A

-20°C

Gel Pack

Lyophilized

Lyophilized from 0.22 μm filtered solution in 50 mM Tris-HCl and 150 mM NaCl, pH 8.0. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

N/A

Centrifuge the vial prior to opening.

For Research Use Only! Not to be used in humans

CTS protease activity also measured by zymograph electrophoresis of Cathepsins. This cathepsin is supplied in 1.

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Recombinants or rec. proteins

recombinants