Human CellExp™ Kallikrein-1, human recombinant

Catalog number
7414-10
Name
Human CellExp™ Kallikrein-1, human recombinant
Size
10 μg
Price
278.00 EUR
Supplier

Details

Target
Kallikrein-1
Background
Kallikrein-1 (KLK1) belongs to the peptidase S1 family and Kallikrein subfamily. KLK1 contains one peptidase S1 domain. KLK-1 preferential cleavage of Arg-|-Xaa bonds in small molecule substrates and also has highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa. Glandular Kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.
Concentration
N/A
Molecular weight
This protein is fused with polyhistidine tag at the C-terminus, has a calculated MW of 27.9 kDa. The predicted N-terminus is Pro 19. DTT-reduced Protein migrates as 40-45 kDa due to glycosylation.
Synonym
KLK1, Kallikrein-1, KLKR, Klk6, hK1
Other name
KLK1, Kallikrein-1, KLKR, Klk6, hK1
NCBI gene number
3816
NCBI gene
KLK1
Gene source
Human
NCBI number
P06870
Recombinant
Yes
Source
HEK293 cells
Purification
≥95%
Tested applications
SDS-PAGE, N/A
Tested activity
N/A
Biological activity
Measured by its ability to cleave a fluorogenic peptide substrate Pro – Phe – Arg – 7 – amido – 4 - methylcoumarin (PFR-AMC). The specific activity is>1200 pmol/min/µg.
Reesults
Measured by its ability to cleave a fluorogenic peptide substrate Pro – Phe – Arg – 7 – amido – 4 - methylcoumarin (PFR-AMC). The specific activity is>1200 pmol/min/µg.
Binding ability
N/A
Unit
N/A
Storage condition
-20°C
Shipping under
Gel Pack
Physical appearance
Lyophilized
Physical properties
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 2 mM CaCl2, 150 mM NaCl, pH 7.5. Normally Mannitol or Trehalose is added as protectants before lyophilization.
How to reconstitute
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants