Human CellExp™ Kallikrein-3, human recombinant

Catalog number
7415-10
Name
Human CellExp™ Kallikrein-3, human recombinant
Size
10 μg
Price
289.00 EUR
Supplier

Details

Target
Kallikrein-3
Background
Kallikrein-3 (KLK3) is also known as Prostate-specific antigen (PSA), APS, Gamma-seminoprotein (Seminin), P-30 antigen, Semenogelase, is a glycoprotein enzyme, is a member of the kallikrein-related peptidase family and is secreted by the epithelial cells of the prostate gland. The preferential cleavage site of KLK3 is “-Tyr-|-Xaa-“, the catalytic activity is inhibited by SERPINA5 and is strongly inhibited by Zn2+. KLK3 hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.
Concentration
N/A
Molecular weight
This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 27.7 kDa. The predicted N-terminus is Ala 18. DTT-reduced Protein migrates as 36 kDa due to glycosylation.
Synonym
PSA, KLK3, KLKB1, Kallikrein-3, APS, KLK2A1, PSA, Hk3, Seminin, Semenogelase
Other name
PSA, KLK3, KLKB1, Kallikrein-3, APS, KLK2A1, PSA, Hk3, Seminin, Semenogelase
NCBI gene number
3818
NCBI gene
KLK3
Gene source
Human
NCBI number
P03952
Recombinant
Yes
Source
HEK293 cells
Purification
≥92%
Tested applications
SDS-PAGE, N/A
Tested activity
N/A
Biological activity
Measured by its ability to cleave the colorimetric peptide substrate, Succinyl – Arg – Pro – Tyr – p – Nitroanilide (Suc-RPY-pNA). The specific activity is>60 pmoles / min / μg.
Reesults
Measured by its ability to cleave the colorimetric peptide substrate, Succinyl – Arg – Pro – Tyr – p – Nitroanilide (Suc-RPY-pNA). The specific activity is>60 pmoles / min / μg.
Binding ability
N/A
Unit
N/A
Storage condition
-20°C
Shipping under
Gel Pack
Physical appearance
Lyophilized
Physical properties
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH 7.5. Normally Mannitol or Trehalose is added as protectants before lyophilization.
How to reconstitute
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants