Human CellExp™ Kallikrein-4, human recombinant

Catalog number
7416-10
Name
Human CellExp™ Kallikrein-4, human recombinant
Size
10 μg
Price
278.00 EUR
Supplier

Details

Target
Kallikrein-4
Background
Kallikrein-4 (KLK4) is also known as Enamel matrix serine proteinase 1 (EMSP1), Kallikrein-like protein 1 (KLK-L1), Prostase, Serine protease 17 (PRSS17), PSTS, which belongs to the peptidase S1 family and Kallikrein subfamily. KLK4 contains one peptidase S1 domain. KLK4 is expressed in prostate and involved in enamel formation. Defects in Kallikrein-4 / KLK4 are the cause of amelogenesis imperfecta hypomaturation type 2A1 (AI2A1) which is an autosomal recessive defect of enamel formation. The disorder involves both primary and secondary dentitions.
Concentration
N/A
Molecular weight
This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 25.2 kDa. The predicted N-terminus is Ser 27. DTT-reduced Protein migrates as 30-32 kDa due to glycosylation.
Synonym
KLK4, Kallikrein-4, Prostate, KLK-L1, EMSP1, PRSS17, PSTS
Other name
KLK4, Kallikrein-4, Prostate, KLK-L1, EMSP1, PRSS17, PSTS
NCBI gene number
9622
NCBI gene
KLK4
Gene source
Human
NCBI number
Q9Y5K2
Recombinant
Yes
Source
HEK293 cells
Purification
≥92%
Tested applications
SDS-PAGE, N/A
Tested activity
N/A
Biological activity
Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC. The specific activity is>200 pmoles / min / μg.
Reesults
Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC. The specific activity is>200 pmoles / min / μg.
Binding ability
N/A
Unit
N/A
Storage condition
-20°C
Shipping under
Gel Pack
Physical appearance
Lyophilized
Physical properties
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH 7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.
How to reconstitute
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants