Human CellExp™ PD-L1 /CD274 /B7-H1, human recombinant

Catalog number
7429-10
Name
Human CellExp™ PD-L1 /CD274 /B7-H1, human recombinant
Size
10 μg
Price
278.00 EUR
Supplier

Details

Target
PD-L1 /CD274 /B7-H1
Background
Programmed cell death 1 ligand 1 (PD-L1) is also known as cluster of differentiation (CD274) or B7 homolog 1 (B7-H1), is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses. B7-H1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. PD-L1 is highly expressed in the heart, skeletal muscle, placenta and lung and weakly expressed in the thymus, spleen, kidney and liver. PD-L1 is expressed on activated T-cells, B-cells, dendritic cells, keratinocytes and monocytes. PD-L1 is up-regulated on T- and B-cells, dendritic cells, keratinocytes and monocytes after LPS and IFNG activation and up-regulated in B-cells activated by surface Ig cross-linking. PD-L1 involve in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner.
Concentration
N/A
Molecular weight
This protein is fused with polyhistidine tag at the C-terminus, has a calculated MW of 26 kDa. The predicted N-terminus is Phe 19. DTT-reduced Protein migrates as 35-42 kDa due to glycosylation.
Synonym
PD-L1, CD274, B7-H1, PDCD1L1, PDCD1LG1
Other name
PD-L1, CD274, B7-H1, PDCD1L1, PDCD1LG1
NCBI gene number
29126
NCBI gene
CD274
Gene source
Human
NCBI number
Q9NZQ7
Recombinant
Yes
Source
HEK293 cells
Purification
≥95%
Tested applications
SDS-PAGE, N/A
Tested activity
N/A
Biological activity
Measured by its binding ability in a functional ELISA. Immobilized rh PD-L1 / B7-H1 (Catalog # 7429) at 2 μg/ml (100 μl/well) can bind rhPD1/PDCD1 Fc Chimera with a linear range of 1-10 μg/ml. Measured by its ability to inhibit anti­CD3 antibody induced IL­2 secretion in human T lymphocytes. The ED50 for this effect is typically 0.6-5 μg/ml.
Reesults
Measured by its binding ability in a functional ELISA. Immobilized rh PD-L1 / B7-H1 (Catalog # 7429) at 2 μg/ml (100 μl/well) can bind rhPD1/PDCD1 Fc Chimera with a linear range of 1-10 μg/ml. Measured by its ability to inhibit anti­CD3 antibody induced IL­2 secretion in human T lymphocytes. The ED50 for this effect is typically 0.6-5 μg/ml.
Binding ability
N/A
Unit
N/A
Storage condition
-20°C
Shipping under
Gel Pack
Physical appearance
Lyophilized
Physical properties
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
How to reconstitute
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants