Human CellExp™ CD23, human recombinant

Catalog number
7435-10
Name
Human CellExp™ CD23, human recombinant
Size
10 μg
Price
262.00 EUR
Supplier

Details

Target
CD23
Background
Cluster of differentiation 23 (CD23), also known as Low affinity immunoglobulin epsilon Fc receptor (FCER2), C-type lectin domain family 4 member J (CLEC4J), Fc-epsilon-RII (FcεRII), Immunoglobulin E-binding factor (IGEBF), is the "low-affinity" receptor for IgE, an antibody isotype involved in allergy and resistance to parasites, and is important in regulation of IgE levels. Unlike many of the antibody receptors, CD23 is a C-type lectin. It is found on mature B cells, activated macrophages, eosinophils, follicular dendritic cells, and platelets. There are two forms of CD23: CD23a and CD23b. CD23a is present on follicular B cells, whereas CD23b requires IL-4 to be expressed on T-cells, monocytes, Langerhans cells, eosinophils, and macrophages. CD23 is known to have role of transportation in antibody feedback regulation. Antigen that enters the blood stream is captured by antigen specific IgE antibodies. The IgE immune complexes that are formed bind to CD23 molecules on B cells, and are transported to the B cell follicles of the spleen. The antigen is then transferred from CD23+ B cells to CD11c+ antigen presenting cells. The CD11c+ cells in turn present the antigen to CD4+ T cells, which can lead to an enhanced antibody response. In flow cytometry, CD23 is helpful in the differentiation of chronic lymphocytic leukemia (CD23-positive) from mantle cell leukemia (CD23-negative).
Concentration
N/A
Molecular weight
This protein is fused with a fused with 6×his tag at the N-terminus and has a calculated MW of 33.2 kDa expressed. The predicted N-terminus is Asp48. Protein migrates as 40-44 kDa in reduced SDS-PAGE resulting from glycosylation.
Synonym
FCER2; FCER-2; CD23; CD-23; CD23A; CD-23A; CLEC4J; FCE2; FCE-2; IGEBF; FcεRII; FcεRⅡ; Fc epsilon RII
Other name
FCER2; FCER-2; CD23; CD-23; CD23A; CD-23A; CLEC4J; FCE2; FCE-2; IGEBF; FcεRII; FcεRⅡ; Fc epsilon RII
NCBI gene number
2208
NCBI gene
FCER2
Gene source
Human
NCBI number
P06734
Recombinant
Yes
Source
HEK293 cells
Purification
≥95%
Tested applications
SDS-PAGE, N/A
Tested activity
N/A
Biological activity
Measured by its binding ability in a functional ELISA. Immobilized rhCD23 at 2 μg/ml (100 μl/well) can bind human IgE with a linear range of 0.01-1.2 μg/ml.
Reesults
Measured by its binding ability in a functional ELISA. Immobilized rhCD23 at 2 μg/ml (100 μl/well) can bind human IgE with a linear range of 0.01-1.2 μg/ml.
Binding ability
N/A
Unit
N/A
Storage condition
-20°C
Shipping under
Gel Pack
Physical appearance
Lyophilized
Physical properties
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
How to reconstitute
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants