Human CellExp™ CD166/ ALCAM, human recombinant

Catalog number

7437-10

Size

10 μg

Price

246.00 EUR

Supplier

Biovision

Order

Details

Target

CD166/ ALCAM

Background

CD166 antigen is a 100-105 kDa type I transmembrane glycoprotein that is a member of the immunoglobulin superfamily of proteins, which is also known as activated leukocyte cell adhesion molecule (ALCAM) in human, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. CD166 is a cell adhesion molecule that binds to CD6. CD166 involved in neurite extension by neurons via heterophilic and homophilic interactions.

Concentration

N/A

Molecular weight

This protein is fused with 6×his tag at the C-terminus, and has a calculated MW of 56.8 kDa. The predicted N-terminus is Trp 28. DTT-reduced Protein migrates as 65-80 kDa in SDS-PAGE due to glycosylation.

Synonym

CD166, ALCAM, CD6L, KG-CAM, MEMD

Other name

CD166, ALCAM, CD6L, KG-CAM, MEMD

NCBI gene number

214

NCBI gene

ALCAM

Gene source

Human

NCBI number

Q13740

Recombinant

Yes

Source

HEK293 cells

Purification

≥95%

Tested applications

SDS-PAGE, N/A

Tested activity

N/A

Biological activity

Measured by its binding ability in a functional ELISA. Immobilized rh CD166 / ALCAM at 10 μg/ml (100 μl/well) can bind mouse CD6 Fc Chimera. The EC50 of mouse CD6 Fc Chimera is 0.1-0.22 μg/ml.

Reesults

Measured by its binding ability in a functional ELISA. Immobilized rh CD166 / ALCAM at 10 μg/ml (100 μl/well) can bind mouse CD6 Fc Chimera. The EC50 of mouse CD6 Fc Chimera is 0.1-0.22 μg/ml.

Binding ability

N/A

Unit

N/A

Storage condition

-20°C

Shipping under

Gel Pack

Physical appearance

Lyophilized

Physical properties

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.

How to reconstitute

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Aa sequence

N/A

Before use

Centrifuge the vial prior to opening.

Notes

For Research Use Only! Not to be used in humans

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Additional source

Recombinants or rec. proteins

Group

recombinants