Human CellExp™ CD166/ ALCAM, human recombinant

Catalog number
7437-50
Name
Human CellExp™ CD166/ ALCAM, human recombinant
Size
50 μg
Price
692.00 EUR
Supplier

Details

Target
CD166/ ALCAM
Background
CD166 antigen is a 100-105 kDa type I transmembrane glycoprotein that is a member of the immunoglobulin superfamily of proteins, which is also known as activated leukocyte cell adhesion molecule (ALCAM) in human, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. CD166 is a cell adhesion molecule that binds to CD6. CD166 involved in neurite extension by neurons via heterophilic and homophilic interactions.
Concentration
N/A
Molecular weight
This protein is fused with 6×his tag at the C-terminus, and has a calculated MW of 56.8 kDa. The predicted N-terminus is Trp 28. DTT-reduced Protein migrates as 65-80 kDa in SDS-PAGE due to glycosylation.
Synonym
CD166, ALCAM, CD6L, KG-CAM, MEMD
Other name
CD166, ALCAM, CD6L, KG-CAM, MEMD
NCBI gene number
214
NCBI gene
ALCAM
Gene source
Human
NCBI number
Q13740
Recombinant
Yes
Source
HEK293 cells
Purification
≥95%
Tested applications
SDS-PAGE, N/A
Tested activity
N/A
Biological activity
Measured by its binding ability in a functional ELISA. Immobilized rh CD166 / ALCAM at 10 μg/ml (100 μl/well) can bind mouse CD6 Fc Chimera. The EC50 of mouse CD6 Fc Chimera is 0.1-0.22 μg/ml.
Reesults
Measured by its binding ability in a functional ELISA. Immobilized rh CD166 / ALCAM at 10 μg/ml (100 μl/well) can bind mouse CD6 Fc Chimera. The EC50 of mouse CD6 Fc Chimera is 0.1-0.22 μg/ml.
Binding ability
N/A
Unit
N/A
Storage condition
-20°C
Shipping under
Gel Pack
Physical appearance
Lyophilized
Physical properties
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
How to reconstitute
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants