Human CellExp™ CXADR /CAR, human recombinant

Catalog number
7441-10
Name
Human CellExp™ CXADR /CAR, human recombinant
Size
10 μg
Price
246.00 EUR
Supplier

Details

Target
CXADR /CAR
Background
Coxsackie virus and adenovirus receptor (CXADR) also known as CAR, is a type I transmembrane glycoprotein for group B coxsackie viruses and subgroup C adenoviruses, and belongs to the CTX family of the Ig superfamily. CAR is strongly expressed in the developing central nervous system. It functions as a homophilic and also as a heterophilic cell adhesion molecule through its interactions with extracellular matrix glycoproteins such as: fibronectin, agrin, laminin-1 and tenascin-R. Human CXADR protein contains a signal sequence, a extracellular domain (ECD) with a V­ type (D1) and a C2­ type (D2) Ig­like domain, a transmembrane segment and a intracellular domain. D1 is thought to be responsible for homodimer formation in trans within tight junctions, and is necessary and sufficient for adenovirus binding. Variants of CXADR are attached to the cell membrane by a GPI- anchor.
Concentration
N/A
Molecular weight
This protein is fused with a C-terminal 6×His tag, has a calculated MW of 24.9 kDa expressed. The predicted N-terminus is Leu 20. Protein migrates as 35 kDa in reduced SDS-PAGE due to glycosylation.
Synonym
CXADR, CAR, CAR4/6, HCAR
Other name
CXADR, CAR, CAR4/6, HCAR
NCBI gene number
1525
NCBI gene
CXADR
Gene source
Human
NCBI number
P78310
Recombinant
Yes
Source
HEK293 cells
Purification
≥95%
Tested applications
SDS-PAGE, N/A
Tested activity
N/A
Biological activity
Measured by the ability of the immobilized protein to support the adhesion of mouse neutrophils. When 50000 cells/well are added to CXADR coated plates (4 μg/mL and 100 μL/well), approximately 20% - 50% will adhere specifically after 60 minutes at 37℃.
Reesults
Measured by the ability of the immobilized protein to support the adhesion of mouse neutrophils. When 50000 cells/well are added to CXADR coated plates (4 μg/mL and 100 μL/well), approximately 20% - 50% will adhere specifically after 60 minutes at 37℃.
Binding ability
N/A
Unit
N/A
Storage condition
-20°C
Shipping under
Gel Pack
Physical appearance
Lyophilized
Physical properties
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
How to reconstitute
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants