Human CellExp™ CNTN2/Contactin-2, human recombinant

Catalog number
7442-50
Name
Human CellExp™ CNTN2/Contactin-2, human recombinant
Size
50 μg
Price
697.00 EUR
Supplier

Details

Target
CNTN2/Contactin-2
Background
Contactin-2 also known as CNTN2, TAX1 (transiently-expressed axonal glycoprotein), TAG1 (transient axonal glycoprotein), and axonin-1, and is a member of the immunoglobulin superfamily. CNTN2 consists of six Ig-like domains and four fibronectin type III domains, and is anchored to the membrane by glycosylphosphatidylinositol (GPI), whereas the soluble form can be released by a GPI-specific phospholipase. As a neural cell adhesion molecule expressed by a subset of neuronal populations in the developing CNS and PNS, CNTN2 mediates cell-cell interactions either via homophilic, or heterophilic contacts with various adhesion molecules including NgCAM, NrCAM, NCAM and neurocan. It is a glycosylphosphatidylinositol (GPI)-anchored neuronal membrane protein that functions as a cell adhesion molecule. It may play a role in the formation of axon connections in the developing nervous system. It may also be involved in glial tumorigenesis and may provide a potential target for therapeutic intervention.
Concentration
N/A
Molecular weight
This protein is fused with 6×his tag at the C-terminus and has a calculated MW of 109 kDa expressed. The predicted N-terminus is Ser 31. Protein migrates as 140 kDa in reduced SDS-PAGE resulting from glycosylation.
Synonym
CNTN2, CNTN-2, AXT, DKFZp781D102, FLJ37193, FLJ42746, MGC157722, TAG-1, TAG1, TAX, TAX1, TAX-1, Contactin-2
Other name
CNTN2, CNTN-2, AXT, DKFZp781D102, FLJ37193, FLJ42746, MGC157722, TAG-1, TAG1, TAX, TAX1, TAX-1, Contactin-2
NCBI gene number
6900
NCBI gene
CNTN2
Gene source
Human
NCBI number
Q02246
Recombinant
Yes
Source
HEK293 cells
Purification
≥95%
Tested applications
SDS-PAGE, N/A
Tested activity
N/A
Biological activity
Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Optimal neurite outgrowth was observed when neurons were plated on 96-well culture plates that had been precoated with 50 μl/well of the rhContactin2 solution at 8-30 μg/ml.
Reesults
Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Optimal neurite outgrowth was observed when neurons were plated on 96-well culture plates that had been precoated with 50 μl/well of the rhContactin2 solution at 8-30 μg/ml.
Binding ability
N/A
Unit
N/A
Storage condition
-20°C
Shipping under
Gel Pack
Physical appearance
Lyophilized
Physical properties
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
How to reconstitute
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants