Human CellExp™ Fas Ligand/FasL, human recombinant

Catalog number
7443-10
Name
Human CellExp™ Fas Ligand/FasL, human recombinant
Size
10 μg
Price
414.00 EUR
Supplier

Details

Target
Fas Ligand/FasL
Background
Fas ligand also known as FasL, CD178, CD95L, or TNFSF6, is a homotrimeric type-II transmembrane protein that belongs to the tumor necrosis factor (TNF) family. Its binding with its receptor induces apoptosis. Fas ligand/receptor interactions play an important role in the regulation of the immune system and the progression of cancer. Mature human Fas Ligand consists of a 179 amino acid (aa) extracellular domain (ECD), a 22 aa transmembrane segment, and a 80 aa cytoplasmic domain. Within the ECD, human Fas Ligand shares 81% and 78% aa sequence identity with mouse and rat Fas Ligand, respectively. Apoptosis triggered by Fas-Fas ligand binding plays a fundamental role in the regulation of the immune system. Its functions include T-cell homeostasis, cytotoxic T-cell activity, immune privilege, maternal tolerance, tumor counterattack. Defective Fas-mediated apoptosis may lead to oncogenesis as well as drug resistance in existing tumors. Germline mutation of Fas is associated with autoimmune lympho proliferative syndrome (ALPS), a childhood disorder of apoptosis.
Concentration
N/A
Molecular weight
This protein is fused with 6×His tag at N-terminus, has a calculated MW of 17.7 kDa. The predicted N-terminus is His. Protein migrates as 25-32 kDa in reduced SDS-PAGE due to glycosylation.
Synonym
FASLG, ALPS1B, APT1LG1, CD178, CD95-L, CD95L, FASL, TNFSF6, Fas ligand
Other name
FASLG, ALPS1B, APT1LG1, CD178, CD95-L, CD95L, FASL, TNFSF6, Fas ligand
NCBI gene number
356
NCBI gene
FASLG
Gene source
Human
NCBI number
P48023
Recombinant
Yes
Source
HEK293 cells
Purification
≥95%
Tested applications
SDS-PAGE, N/A
Tested activity
N/A
Biological activity
Measured by its ability to induce apoptosis of Jurkat human acute T cell leukemia cells. The ED50 for this effect is typically 0.1-1.5 ng/mL in the presence of 10 μg/mL of a crosslinking antibody Mouse Anti poly-Histidine Monoclonal Antibody.
Reesults
Measured by its ability to induce apoptosis of Jurkat human acute T cell leukemia cells. The ED50 for this effect is typically 0.1-1.5 ng/mL in the presence of 10 μg/mL of a crosslinking antibody Mouse Anti poly-Histidine Monoclonal Antibody.
Binding ability
N/A
Unit
N/A
Storage condition
-20°C
Shipping under
Gel Pack
Physical appearance
Lyophilized
Physical properties
Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH 7.0. Normally Mannitol or Trehalose is added as protectants before lyophilization.
How to reconstitute
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Description
FAS ligand and other ligands are binding to the receptor for signaling pathways for example in apoptosis or JNK signaling. Receptor agonists are often tested for drug development.
Additional source
Recombinants or rec. proteins
Group
recombinants