Human CellExp™ LIFR /CD118, human recombinant

Catalog number
7444-100
Name
Human CellExp™ LIFR /CD118, human recombinant
Size
100 μg
Price
697.00 EUR
Supplier

Details

Target
LIFR /CD118
Background
Leukemia inhibitory factor receptor also known as LIFR; CD118; FLJ98106; FLJ99923; LIF-R; SJS2; STWS; SWS, is the receptor for leukemia inhibitory factor (LIF). The leukemia inhibitory factor is a polyfunctional cytokine that affects the differentiation, survival, and proliferation of a wide variety of cells in the adult and the embryo. LIF action appears to be mediated through a high-affinity receptor complex composed of a low-affinity LIF binding chain (LIF receptor) and a high-affinity converter subunit, gp130. Both LIFR and gp130 are members of a family of cytokine receptors that includes components of the receptors for the majority of hematopoietic cytokines and for cytokines that affect other systems, including the ciliary neurotrophic factor, growth hormone and prolactin Defects in LIFR are the cause of Stueve-Wiedemann syndrome (SWS), a severe autosomal recessive condition and belongs to the group of the bent-bone dysplasias.
Concentration
N/A
Molecular weight
This protein is fused with the Fc region of human IgG1 at the CT. The reduced monomer has a calculated molecular mass of 115.5 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhLIFR-Fc monomer is approximately 135-150 kDa due to the glycosylation.
Synonym
LIFR, CD118, FLJ98106, FLJ99923, LIF-R, SJS2, STWS, SWS, Leukemia inhibitory factor receptor, Cluster of Differentiation 118
Other name
LIFR, CD118, FLJ98106, FLJ99923, LIF-R, SJS2, STWS, SWS, Leukemia inhibitory factor receptor, Cluster of Differentiation 118
NCBI gene number
3977
NCBI gene
LIFR
Gene source
Human
NCBI number
P42702
Recombinant
Yes
Source
HEK293 cells
Purification
≥96%
Tested applications
SDS-PAGE, N/A
Tested activity
N/A
Biological activity
Measured by its ability to inhibit LIF-dependent proliferation of TF1 human erythroleukemic cells. The ED50 for this effect is typically 5-10 μg/ml in the presence of 0.3 ng/ml of recombinant human LIF.
Reesults
Measured by its ability to inhibit LIF-dependent proliferation of TF1 human erythroleukemic cells. The ED50 for this effect is typically 5-10 μg/ml in the presence of 0.3 ng/ml of recombinant human LIF.
Binding ability
N/A
Unit
N/A
Storage condition
-20°C
Shipping under
Gel Pack
Physical appearance
Lyophilized
Physical properties
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
How to reconstitute
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants