Human CellExp™ Neprilysin /CD10, human recombinant

Catalog number
7445-10
Name
Human CellExp™ Neprilysin /CD10, human recombinant
Size
10 μg
Price
414.00 EUR
Supplier

Details

Target
Neprilysin /CD10
Background
Cluster of differentiation 10 (CD10), membrane metallo-endopeptidase, neutral endopeptidase (NEP), Neprilysin, and common acute lymphoblastic leukemia antigen (CALLA), is a 90-110-kDa type II transmembrane glycoprotein normally expressed by a variety of tissues, including epithelial cells of the prostate, kidney, intestine, endometrium, adrenal glands, and lung. This zinc-dependent metalloprotease enzyme cleaves peptide bonds on the amino side of hydrophobic residues and inactivates a variety of physiologically active secreted peptides. CD20 is thought to be the rate-limiting degrading enzyme of amyloid β peptide (Aβ) whose abnormal misfolding and aggregation in neural tissue has been implicated in the development of Alzheimer's disease (AD). CD10 is also identified as the common acute lymphoblastic leukemia antigen (CALLA) present on leukemic cells of pre-B phenotype, and thus serves as the most important biomarker in the diagnosis of human acute lymphocytic leukemia (ALL).
Concentration
N/A
Molecular weight
This protein with Gly-Pro at the N- terminus, has a calculated MW of 80 kDa. The predicted N-terminus is Tyr 52. DTT-reduced Protein migrates as 90-100 kDa due to glycosylation.
Synonym
MME, CALLA, CD10, DKFZp686O16152, MGC126681, MGC126707, NEP, SFE, Neprilysin
Other name
MME, CALLA, CD10, DKFZp686O16152, MGC126681, MGC126707, NEP, SFE, Neprilysin
NCBI gene number
4311
NCBI gene
MME
Gene source
Human
NCBI number
P08473
Recombinant
Yes
Source
HEK293 cells
Purification
≥95%
Tested applications
SDS-PAGE, N/A
Tested activity
N/A
Biological activity
Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK-(Dnp)-OH. The specific activity is>2500 pmol/min/μg.
Reesults
Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK-(Dnp)-OH. The specific activity is>2500 pmol/min/μg.
Binding ability
N/A
Unit
N/A
Storage condition
-20°C
Shipping under
Gel Pack
Physical appearance
Lyophilized
Physical properties
Lyophilized from 0.22 μm filtered solution in 50 mM tris, 150 mM NaCl, pH 8.0 Normally Mannitol or Trehalose is added as protectants before lyophilization.
How to reconstitute
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants