Human CellExp™ Decorin /Bone proteoglycan II, human recombinant

Catalog number
7449-20
Name
Human CellExp™ Decorin /Bone proteoglycan II, human recombinant
Size
20 μg
Price
273.00 EUR
Supplier

Details

Target
Decorin /Bone proteoglycan II
Background
Decorin, also known as bone proteoglycan II, PGS2, SLRR1B, DCN, DSPG2 and PG40, is a secreted chondroitin /dermatan sulfate proteoglycan in the family of small leucine-rich proteoglycans (SLRPs). Decorin is a small cellular or pericellular matrix proteoglycan and is closely related in structure to biglycan protein. Decorin and biglycan are thought to be the result of gene duplication. This protein is a component of connective tissue, binds to type I collagen fibrils, and plays a role in matrix assembly. Decorin appears to influence fibrillogenesis, and also interacts with fibronectin, thrombospondin, the complement component C1q, epidermal growth factor receptor (EGFR) and transforming growth factor-beta (TGF-beta). Defects in DCN are the cause of congenital stromal corneal dystrophy (CSCD).
Concentration
N/A
Molecular weight
This protein fused with 6×His tag at the C-terminus, has a calculated MW of 38.8 kDa. The predicted N-terminus is Gly 17. DTT-reduced Protein migrates as 45 kDa or higher due to different glycosylation.
Synonym
Decorin, DCN, CSCD, DSPG2, PG40, PGII, PGS2, SLRR1B.
Other name
Decorin, DCN, CSCD, DSPG2, PG40, PGII, PGS2, SLRR1B.
NCBI gene number
1634
NCBI gene
DCN
Gene source
Human
NCBI number
P07585
Recombinant
Yes
Source
HEK293 cells
Purification
≥95%
Tested applications
SDS-PAGE, N/A
Tested activity
N/A
Biological activity
Measured by its ability to modulate collagen fibrillogenesis. At 5 µg/mL, rh Decorin can significantly delay the rate of type I collagen fibrillogenesis.
Reesults
Measured by its ability to modulate collagen fibrillogenesis. At 5 µg/mL, rh Decorin can significantly delay the rate of type I collagen fibrillogenesis.
Binding ability
N/A
Unit
N/A
Storage condition
-20°C
Shipping under
Gel Pack
Physical appearance
Lyophilized
Physical properties
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
How to reconstitute
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Tissue
bone
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants