Human CellExp™ FOLR1, human recombinant

Catalog number
7456-10
Name
Human CellExp™ FOLR1, human recombinant
Size
10 μg
Price
246.00 EUR
Supplier

Details

Target
FOLR1
Background
Folate Receptor 1 (FOLR1) also known as Folate receptor alpha, Folate Binding Protein (FBP), FOLR, and is a member of the folate receptor (FOLR) family. Members of this gene family have a high affinity for folic acid and for several reduced folic acid derivatives, and mediate delivery of 5-methyltetrahydrofolate to the interior of cells. Mature FOLR1 is an N-glycosylated protein that is anchored to the cell surface by a GPI linkage. FOLR1 is predominantly expressed on epithelial cells and is dramatically upregulated on many carcinomas. FOLR1 is internalized to the endosomal system where it dissociates from its ligand before recycling to the cell surface. A soluble form of FOLR1 can be proteolytically shed from the cell surface into the serum and breast milk. Defects in FOLR1 are the cause of neurodegeneration due to cerebral folate transport deficiency (NCFTD). NCFTD is an autosomal recessive disorder resulting from brain-specific folate deficiency early in life.
Concentration
N/A
Molecular weight
This protein is fused with 6×his tag at the C-terminus and has a calculated MW of 25.4 kDa expressed. The predicted N-terminus is Arg 25. Protein migrates as 33-37 kDa in reduced SDS-PAGE resulting from glycosylation.
Synonym
FOLR1, FBP, FOLR, FOLR-1, Folate-receptor-alpha
Other name
FOLR1, FBP, FOLR, FOLR-1, Folate-receptor-alpha
NCBI gene number
2348
NCBI gene
FOLR1
Gene source
Human
NCBI number
P15328
Recombinant
Yes
Source
HEK293 cells
Purification
≥95%
Tested applications
SDS-PAGE, N/A
Tested activity
N/A
Biological activity
Measured by its binding ability in a functional ELISA. When Folic Acid Bovine Serum Albumin is coated at 5 μg/ml (100 μl/well), the concentration of rhFOLR1 that produces 50% of the optimal binding response is found to be approximately 0.35-1.8 nM.
Reesults
Measured by its binding ability in a functional ELISA. When Folic Acid Bovine Serum Albumin is coated at 5 μg/ml (100 μl/well), the concentration of rhFOLR1 that produces 50% of the optimal binding response is found to be approximately 0.35-1.8 nM.
Binding ability
N/A
Unit
N/A
Storage condition
-20°C
Shipping under
Gel Pack
Physical appearance
Lyophilized
Physical properties
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
How to reconstitute
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants