Human CellExp™ SFRP1/SARP2, human recombinant

Catalog number
7458-10
Name
Human CellExp™ SFRP1/SARP2, human recombinant
Size
10 μg
Price
262.00 EUR
Supplier

Details

Target
SFRP1/SARP2
Background
Secreted frizzled-related protein 1 (SFRP1) also known as Secreted apoptosis-related protein 2 (SARP2), FRP, FRP1, is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. The Secreted frizzled-related protein (SFRP) family consists of five secreted glycoproteins in humans (SFRP1, SFRP2, SFRP3, SFRP4, SFRP5) that act as extracellular signaling ligands. SFRP1 / SARP-2 is widely expressed and absent from lung, liver and peripheral blood leukocytes, and with the highest levels in heart and fetal kidney. SARP2 / SFRP-1 is a 35 kDa prototypical member of the SFRP family. SARP2 / FRP-1 acts as a biphasic modulator of Wnt signaling, counteracting Wnt-induced effects at high concentrations and promoting them at lower concentrations.
Concentration
N/A
Molecular weight
This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 33.4 kDa. The predicted N-terminus is Ser 32. DTT-reduced Protein migrates as 38 kDa due to glycosylation.
Synonym
SFRP1, FRP, FRP1, SARP2, FrzA
Other name
SFRP1, FRP, FRP1, SARP2, FrzA
NCBI gene number
6422
NCBI gene
SFRP1
Gene source
Human
NCBI number
Q8N474
Recombinant
Yes
Source
HEK293 cells
Purification
≥92%
Tested applications
SDS-PAGE, N/A
Tested activity
N/A
Biological activity
Measured by its ability to inhibit proliferation of HeLa human cervical epithelial carcinoma cells. The ED50 for this effect is typically 2.5 - 10 µg/ml.
Reesults
Measured by its ability to inhibit proliferation of HeLa human cervical epithelial carcinoma cells. The ED50 for this effect is typically 2.5 - 10 µg/ml.
Binding ability
N/A
Unit
N/A
Storage condition
-20°C
Shipping under
Gel Pack
Physical appearance
Lyophilized
Physical properties
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
How to reconstitute
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants