Human CellExp™ LRRC4 /NGL-2, human recombinant

Catalog number
7461-50
Name
Human CellExp™ LRRC4 /NGL-2, human recombinant
Size
50 μg
Price
719.00 EUR
Supplier

Details

Target
LRRC4 /NGL-2
Background
LRRC4 (Leu-rich repeat/LRR-containing glycoprotein 4), LRRC4B and LRRC4C are post-synaptic adhesion molecules of the LRR protein family that induce excitatory synapse formation. LRRC4 is also known as Brain tumor-associated protein BAG, Netrin-G2 ligand (NGL-2), Nasopharyngeal carcinoma-associated gene 14 protein (NAG14), which contains 1 Ig-like (immunoglobulin-like) domain and 9 LRR (leucine-rich) repeats, 1 LRRCT domain, 1 LRRNT domain. LRRC4 / NGL-2 specifically expressed in brain. LRRC4 / NGL-2 regulates the formation of excitatory synapses through the recruitment of pre-and-postsynaptic proteins. LRRC4 / NGL-2 organize the lamina/pathway-specific differentiation of dendrites. LRRC4 / NGL-2 plays an important role for auditory synaptic responses and involved in the suppression of glioma.
Concentration
N/A
Molecular weight
This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 55.6 kDa. The predicted N-terminus is Ala 39. DTT-reduced Protein migrates as 90-110 kDa due to glycosylation.
Synonym
LRRC4, NGL-2, BAG, NAG14.
Other name
LRRC4, NGL-2, BAG, NAG14.
NCBI gene number
64101
NCBI gene
LRRC4
Gene source
Human
NCBI number
Q9HBW1
Recombinant
Yes
Source
HEK293 cells
Purification
≥92%
Tested applications
SDS-PAGE, N/A
Tested activity
N/A
Biological activity
Measured by its binding ability in a functional ELISA. Immobilized Recombinant mouse Netrin-G2a Fc chimera at 4 μg/ml, can bind rhLRRC4 with an apparent KD <10 nM.
Reesults
Measured by its binding ability in a functional ELISA. Immobilized Recombinant mouse Netrin-G2a Fc chimera at 4 μg/ml, can bind rhLRRC4 with an apparent KD <10 nM.
Binding ability
N/A
Unit
N/A
Storage condition
-20°C
Shipping under
Gel Pack
Physical appearance
Lyophilized
Physical properties
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
How to reconstitute
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants