Human CellExp™ CD155, human recombinant

Catalog number
7462-10
Name
Human CellExp™ CD155, human recombinant
Size
10 μg
Price
246.00 EUR
Supplier

Details

Target
CD155
Background
CD155 is a Type I transmembrane glycoprotein in the immunoglobulin superfamily. Commonly known as Poliovirus Receptor (PVR) due to its involvement in the cellular poliovirus infection in primates, CD155's normal cellular function is in the establishment of intercellular adherens junctions between epithelial cells. CD155/PVR was originally isolated based on its ability to mediate polio virus attachment to host cells. The full length (or CD155 alpha isoform) is synthesized as a 417 amino acid (aa) precursor that contains a 20 aa signal sequence, a 323 aa extracellular region, a 24 aa TM segment and a 50 aa cytoplasmic tail. The extracellular region contains one N terminal V type and two C2 type Ig like domains. It has 3 extracellular immunoglobulin-like domains, D1-D3, where D1 is recognized by the virus. Low resolution structures of CD155 complexed with poliovirus have been obtained using electron microscopy[1] while a high resolution structures of the ectodomain D1 and D2 of CD155 were solved by x-ray crystallography.
Concentration
N/A
Molecular weight
This protein is fused with a C-terminal 6×his tag and has a calculated MW of 28 kDa. The predicted N-terminus is Trp21. DTT-reduced protein migrates as 50-65 kDa polypeptide in SDS-PAGE resulting from glycosylation.
Synonym
PVR, FLJ25946, PVS, CD155, TAGE4, HVED, NECL5, cluster of differentiation 155.
Other name
PVR, FLJ25946, PVS, CD155, TAGE4, HVED, NECL5, cluster of differentiation 155.
NCBI gene number
5817
NCBI gene
PVR
Gene source
Human
NCBI number
P15151
Recombinant
Yes
Source
HEK293 cells
Purification
≥95%
Tested applications
SDS-PAGE, N/A
Tested activity
N/A
Biological activity
Measured by its ability to bind with recombinant human DNAM1 / CD226 in a functional ELISA assay
Reesults
Measured by its ability to bind with recombinant human DNAM1 / CD226 in a functional ELISA assay
Binding ability
N/A
Unit
N/A
Storage condition
-20°C
Shipping under
Gel Pack
Physical appearance
Lyophilized
Physical properties
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
How to reconstitute
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants