Human CellExp™ GFRA1 /GDNFRA, human recombinant

Catalog number
7469-50
Name
Human CellExp™ GFRA1 /GDNFRA, human recombinant
Size
50 μg
Price
719.00 EUR
Supplier

Details

Target
GFRA1 /GDNFRA
Background
GDNF family receptor alpha-1 (GFRA1) also known as RET ligand 1 (RETL1), TGF-beta-related neurotrophic factor receptor 1 (TRNR1), is a novel glycosylphosphatidylinositol (GPI)-linked cell surface receptor, and belongs to the glial cell line-derived neurotrophic factor (GDNF) receptor subfamily. The GDNF family ligands comprises GDNF, Neurturin (NTN), Artemin, and Persephin. GDNF and NTN are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. GFRA1 mediates the association with and activation of the RET tyrosine kinase receptor (RTK), and subsequently initiates the RET signaling pathway. GFR alpha-mediated signaling plays an important role in the survival, differentiation, and migration of central and peripheral neurons, and is essential for the development of kidneys and the enteric nervous system, as well as the inflammatory response in some carcinomas.
Concentration
N/A
Molecular weight
This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 45.5 kDa. The predicted N-terminus is Asp 25. DTT-reduced Protein migrates as 55-60 kDa due to glycosylation.
Synonym
GFRA1, GDNFRA, RETL1, TRNR1, GDNFR, GFR-ALPHA-1, RET1L
Other name
GFRA1, GDNFRA, RETL1, TRNR1, GDNFR, GFR-ALPHA-1, RET1L
NCBI gene number
2674
NCBI gene
GFRA1
Gene source
Human
NCBI number
P56159
Recombinant
Yes
Source
HEK293 cells
Purification
≥95%
Tested applications
SDS-PAGE, N/A
Tested activity
N/A
Biological activity
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human GDNF at 1 µg/mL binds rhGFRA1 with an apparent KD
Reesults
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human GDNF at 1 µg/mL binds rhGFRA1 with an apparent KD
Binding ability
N/A
Unit
N/A
Storage condition
-20°C
Shipping under
Gel Pack
Physical appearance
Lyophilized
Physical properties
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
How to reconstitute
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants