Human CellExp™ GFRA2 /GDNFRB, human recombinant

Catalog number
7470-20
Name
Human CellExp™ GFRA2 /GDNFRB, human recombinant
Size
20 μg
Price
268.00 EUR
Supplier

Details

Target
GFRA2 /GDNFRB
Background
GDNF family receptor alpha-2 (GFRA2) also known as GDNF receptor beta, Neurturin receptor alpha, RET ligand 2, TGF-beta-related neurotrophic factor receptor 2, is a cell membrane protein which belongs to the GDNFR family. Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. GDNF is a glycosylated, disulfide-bonded homodimer that is distantly related to the TGF-beta superfamily of growth factors. Three receptors for these factors, GFRα-1, GFRα-2 and GFRα-3 have been identified. The isoform 1 of GFRA2 is found in both brain and placenta. GFRA2 mediates the NRTN-induced autophosphorylation and activation of the RET receptor and also able to mediate GDNF signaling through the RET tyrosine kinase receptor. GFRA2 mediates the NRTN-induced autophosphorylation and activation of the RET receptor. It can also mediate GDNF signaling through the RET tyrosine kinase receptor.
Concentration
N/A
Molecular weight
This protein is fused with 6×His tag at the N-terminus, has a calculated MW of 47.6 kDa. The predicted N-terminus is Ser 22. DTT-reduced Protein migrates as 60-70 KDa due to glycosylation.
Synonym
GFRA2, GDNFRB, NRTNR-ALPHA, NTNRA, RETL2, TRNR2.
Other name
GFRA2, GDNFRB, NRTNR-ALPHA, NTNRA, RETL2, TRNR2.
NCBI gene number
2675
NCBI gene
GFRA2
Gene source
Human
NCBI number
O00451
Recombinant
Yes
Source
HEK293 cells
Purification
≥95%
Tested applications
SDS-PAGE, N/A
Tested activity
N/A
Biological activity
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Neurturin at 1 µg/ml can bind rhGFRA2 with an apparent KD <8 nM.
Reesults
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Neurturin at 1 µg/ml can bind rhGFRA2 with an apparent KD <8 nM.
Binding ability
N/A
Unit
N/A
Storage condition
-20°C
Shipping under
Gel Pack
Physical appearance
Lyophilized
Physical properties
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
How to reconstitute
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants