Human CellExp™ FOLR2, human recombinant

Catalog number
7471-50
Name
Human CellExp™ FOLR2, human recombinant
Size
50 μg
Price
670.00 EUR
Supplier

Details

Target
FOLR2
Background
Folate receptor beta, also known as Folate receptor 2, FBP, FOLR2, BETA-HFR, FBP/PL-1, FR-BETA, FR-P3, and is a member of the folate receptor (FOLR) family which mediates delivery of 5-methyltetrahydrofolate to the interior of cells. This protein has a 68% and 79% sequence homology with the FOLR1 and FOLR3 proteins, respectively. The FOLR2 protein was originally thought to exist only in placenta, but is also detected in spleen, bone marrow, and thymus. FOLR2 is predominantly expressed in placenta, cells of the neutrophilic lineage, and some CD34+ hematopoietic progenitor cells. It is upregulated on myeloid leukemias, head and neck squamous cell carcinomas, and several nonepithelial cancers. It is also upregulated on macrophages and monocytes at chronic inflammatory sites including rheumatoid arthritis synovium and glioblastoma. FOLR2 is a marker for macrophages generated in the presence of M-CSF, but not GM-CSF. Its expression correlates with increased folate uptake ability. Folate conjugates of therapeutic drugs are a potential immunotherapy tool to target tumor-associated macrophages.
Concentration
N/A
Molecular weight
This protein is fused with 6×his tag at the C-terminus and has a calculated MW of 26 kDa expressed. The predicted N-terminus is Thr17. Protein migrates as 30-33 kDa in reduced SDS-PAGE resulting from glycosylation.
Synonym
FOLR2, FOLR-2, BETA-HFR, FBP/PL-1, FR-BETA, FR-P3, Folate-receptor-beta.
Other name
FOLR2, FOLR-2, BETA-HFR, FBP/PL-1, FR-BETA, FR-P3, Folate-receptor-beta.
NCBI gene number
2350
NCBI gene
FOLR2
Gene source
Human
NCBI number
P14207
Recombinant
Yes
Source
HEK293 cells
Purification
≥95%
Tested applications
SDS-PAGE, N/A
Tested activity
N/A
Biological activity
Measured by its binding ability in a functional ELISA. When Folic Acid Bovine Serum Albumin was coated at 5 μg/ml (100 μl/well), the concentration of rhFOLR2 that produces 50% of the optimal binding response was found to be approximately 0.15-1.1 nM.
Reesults
Measured by its binding ability in a functional ELISA. When Folic Acid Bovine Serum Albumin was coated at 5 μg/ml (100 μl/well), the concentration of rhFOLR2 that produces 50% of the optimal binding response was found to be approximately 0.15-1.1 nM.
Binding ability
N/A
Unit
N/A
Storage condition
-20°C
Shipping under
Gel Pack
Physical appearance
Lyophilized
Physical properties
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
How to reconstitute
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants