Human CellExp™ LDLR, human recombinant

Catalog number
7472-50
Name
Human CellExp™ LDLR, human recombinant
Size
50 μg
Price
828.00 EUR
Supplier

Details

Target
LDLR
Background
Low-Density Lipoprotein (LDL) Receptor, also known as LDLR, FH, FHC, LDLCQ2, and is a mosaic protein of ~840 amino acids (after removal of signal peptide) that mediates the endocytosis of cholesterol-rich LDL. It is a cell-surface receptor that recognizes the apoprotein B100 which is embedded in the phospholipid outer layer of LDL particles. The receptor also recognizes the apoE protein found in chylomicron remnants and VLDL remnants (IDL). It belongs to the Low density lipoprotein receptor gene family. LDL receptor complexes are present in clathrin-coated pits (or buds) on the cell surface, which when bound to LDL-cholesterol via adaptin, are pinched off to form clathrin-coated vesicles inside the cell. This allows LDL-cholesterol to be bound and internalized in a process known as endocytosis and prevents the LDL just diffusing around the membrane surface. This occurs in all nucleated cells (not erythrocytes), but mainly in the liver which removes ~70% of LDL from the circulation. Synthesis of receptors in the cell is regulated by the level of free intracellular cholesterol; if it is in excess for the needs of the cell then the transcription of the receptor gene will be inhibited. LDL receptors are translated by ribosomes on the endoplasmic reticulum and are modified by the Golgi apparatus before travelling in vesicles to the cell surface. LDL is directly involved in the development of atherosclerosis, due to accumulation of LDL-cholesterol in the blood. Atherosclerosis is the process responsible for the majority of cardiovascular diseases.
Concentration
N/A
Molecular weight
This protein is fused with a C-terminal 6×his tag and has a calculated MW of 86 kDa. The predicted N-terminal is Ala22 or Asp193. Corresponding to the mature and immature form, DTT-reduced protein migrates as 95-110 kDa &125-140 kDa polypeptide in SDS-PAGE resulting from different glycosylation.
Synonym
LDLR, FH, FHC, LDLCQ2, Low-Density Lipoprotein (LDL) Receptor
Other name
LDLR, FH, FHC, LDLCQ2, Low-Density Lipoprotein (LDL) Receptor
NCBI gene number
3949
NCBI gene
LDLR
Gene source
Human
NCBI number
P01130
Recombinant
Yes
Source
HEK293 cells
Purification
≥90%
Tested applications
SDS-PAGE, N/A
Tested activity
N/A
Biological activity
Measured in a competitive binding assay. When human LDL is immobilized at 1 μg/ml (100 μl/well), recombinant human LDLR inhibits 50% binding of biotinylated recombinant human LDLR (0.5 μg/ml) at the concentration range of 0.35 – 2.5 μg/ml.
Reesults
Measured in a competitive binding assay. When human LDL is immobilized at 1 μg/ml (100 μl/well), recombinant human LDLR inhibits 50% binding of biotinylated recombinant human LDLR (0.5 μg/ml) at the concentration range of 0.35 – 2.5 μg/ml.
Binding ability
N/A
Unit
N/A
Storage condition
-20°C
Shipping under
Gel Pack
Physical appearance
Lyophilized
Physical properties
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
How to reconstitute
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants