Human CellExp™ Carbonic Anhydrase 4/CA4, human recombinant

Catalog number
7484-10
Name
Human CellExp™ Carbonic Anhydrase 4/CA4, human recombinant
Size
10 μg
Price
300.00 EUR
Supplier

Details

Target
Carbonic Anhydrase 4/CA4
Background
Carbonic anhydrase 4 (CA4) is also known as Carbonate dehydratase IV, Carbonic anhydrase IV, which belongs to the alpha-carbonic anhydrase family. CA4 may stimulate the sodium/bicarbonate transporter activity of SLC4A4 that acts in pH homeostasis. It is essential for acid overload removal from the retina and retina epithelium, and acid release in the choriocapillaris in the choroid. The enzyme regulation is activated by histamine, L-adrenaline, D-phenylalanine, L- and D-histidine. CA4 can interact with SLC4A4.
Concentration
N/A
Molecular weight
This protein is fused with polyhistidine tag at the C-terminus, and has a calculated MW of 31 kDa. The predicted N-terminus is Ala 19. DTT-reduced Protein migrates as 33 kDa in SDS-PAGE.
Synonym
CA4, Carbonic anhydrase 4
Other name
CA4, Carbonic anhydrase 4
NCBI gene number
762
NCBI gene
CA4
Gene source
Human
NCBI number
P22748
Recombinant
Yes
Source
HEK293 cells
Purification
≥92%
Tested applications
SDS-PAGE, N/A
Tested activity
N/A
Biological activity
Measured by its esterase activity. The specific activity is>2 pmol/min/µg.
Reesults
Measured by its esterase activity. The specific activity is>2 pmol/min/µg.
Binding ability
N/A
Unit
N/A
Storage condition
-20°C
Shipping under
Gel Pack
Physical appearance
Lyophilized
Physical properties
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH 7.5. Normally Mannitol or Trehalose is added as protectants before lyophilization.
How to reconstitute
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants