Human CellExp™ Carbonic Anhydrase 10/CA10, human recombinant

Catalog number
7485-10
Name
Human CellExp™ Carbonic Anhydrase 10/CA10, human recombinant
Size
10 μg
Price
268.00 EUR
Supplier

Details

Target
Carbonic Anhydrase 10/CA10
Background
Carbonic anhydrase-related protein 10 (CA10) is also known as Carbonic anhydrase-related protein X (CARP X or CARPX), Cerebral protein 15 (hucep-15), which belongs to the alpha-carbonic anhydrase family of zinc metalloenzymes, which catalyze the reversible hydration of carbon dioxide in various biological processes. CA10 / CARPX is an acatalytic member of the alpha-carbonic anhydrase subgroup, and it is thought to play a role in the central nervous system, especially in brain development.
Concentration
N/A
Molecular weight
This protein is fused with polyhistidine tag at the C-terminus, has a calculated MW of 32.6 kDa. The predicted N-terminus is Gln 22. DTT-reduced Protein migrates as 37 kDa due to glycosylation.
Synonym
CA10, Carbonic Anhydrase X, CARPX, hucep-15
Other name
CA10, Carbonic Anhydrase X, CARPX, hucep-15
NCBI gene number
56934
NCBI gene
CA10
Gene source
Human
NCBI number
Q9NS85
Recombinant
Yes
Source
HEK293 cells
Purification
≥95%
Tested applications
SDS-PAGE, N/A
Tested activity
N/A
Biological activity
Measured by its esterase activity. The specific activity, as measured with 1 mM 4-Nitrophenyl acetate (4-NPA) as substrate and 5 μg rh CA10 / CARPX enzyme at 100 ng/µl in 100 μL of 12.5 mM Tris, 75 mM NaCl, pH 7.5. The specific activity is>1 pmol/min/µg.
Reesults
Measured by its esterase activity. The specific activity, as measured with 1 mM 4-Nitrophenyl acetate (4-NPA) as substrate and 5 μg rh CA10 / CARPX enzyme at 100 ng/µl in 100 μL of 12.5 mM Tris, 75 mM NaCl, pH 7.5. The specific activity is>1 pmol/min/µg.
Binding ability
N/A
Unit
N/A
Storage condition
-20°C
Shipping under
Gel Pack
Physical appearance
Lyophilized
Physical properties
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH 7.5. Normally Mannitol or Trehalose is added as protectants before lyophilization.
How to reconstitute
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants