Human CellExp™ CD85J/LILRB1, human recombinant

Catalog number
7491-50
Name
Human CellExp™ CD85J/LILRB1, human recombinant
Size
50 μg
Price
719.00 EUR
Supplier

Details

Target
CD85J/LILRB1
Background
CD85 antigen-like family member J (CD85J) also known as Leukocyte immunoglobulin-like receptor subfamily B member 1 (LILRB1 or LIR-1), Immunoglobulin-like transcript 2 (ILT-2), Monocyte/macrophage immunoglobulin-like receptor 7 (MIR7), which belongs to leukocyte immunoglobulin-like receptor (LIR) family. CD85J / LILRB1 Contains 4 Ig-like C2-type (immunoglobulin-like) domains. CD85J / LIR-1 is expressed predominantly on B-cells and monocytes. CD85J is receptor for class I MHC antigens and recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles and is also receptor for H301/UL18, a human cytomegalovirus class I MHC homolog. Ligand binding results in inhibitory signals and down-regulation of the immune response. CD85J / LILRB1 interaction with HLA-B or HLA-E leads to inhibition of the signal triggered by FCER1A and inhibits serotonin release. CD85J / LILRB1 inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions.
Concentration
N/A
Molecular weight
This protein is fused with Fc fragment of human IgG1 at the C-terminus, has a calculated MW of 73.8 kDa. The predicted N-terminus is Gly 24. DTT-reduced Protein migrates as 90-110 kDa due to glycosylation.
Synonym
CD85J, LILRB1, CD85, ILT2, LIR1, MIR7
Other name
CD85J, LILRB1, CD85, ILT2, LIR1, MIR7
NCBI gene number
10859
NCBI gene
LILRB1
Gene source
Human
NCBI number
Q8NHL6
Recombinant
Yes
Source
HEK293 cells
Purification
≥95%
Tested applications
SDS-PAGE, N/A
Tested activity
N/A
Biological activity
Measured by its ability to support the adhesion of HSB2 human peripheral blood acute lymphoblastic leukemia cells. Immobilized rhCD85J / LILRB1 Fc Chimera at 10 µg/mL, 100 µL/well can support 60-80% HSB2 cell adhesion, when 100000 cells were added in each well of a 96-well plate.
Reesults
Measured by its ability to support the adhesion of HSB2 human peripheral blood acute lymphoblastic leukemia cells. Immobilized rhCD85J / LILRB1 Fc Chimera at 10 µg/mL, 100 µL/well can support 60-80% HSB2 cell adhesion, when 100000 cells were added in each well of a 96-well plate.
Binding ability
N/A
Unit
N/A
Storage condition
-20°C
Shipping under
Gel Pack
Physical appearance
Lyophilized
Physical properties
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
How to reconstitute
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants