Human CellExp™ PDGFRb/CD140b, human recombinant

Catalog number
7493-10
Name
Human CellExp™ PDGFRb/CD140b, human recombinant
Size
10 μg
Price
246.00 EUR
Supplier

Details

Target
PDGFRb/CD140b
Background
Human platelet-derived growth factor receptor, beta polypeptide (PDGFRB), also called J03278, M21616, CD140B, JTK12, PDGF-R-beta and PDGFR, is receptor that binds specifically to PDGFB and has a tyrosine-protein kinase activity. Is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor (PDGF) family. The PDGFR/PDGF system includes two receptors (PDGFRA and PDGFRB) and four ligands (A, B, C and D). The receptors PDGFRA and PDGFRB are related in sequence and both are members of the class III subtype of receptor tyrosine kinases (RTKs). Other class III RTKs are CSF1R, KIT and FLT3. PDGF binding induces receptor homo- and heterodimerization and signal transduction. The expression of the α and β receptors is independently regulated in various cell types. Recombinant soluble PDGFRB binds PDGF with high affinity and is potent PDGF antagonist. The ligands form either homo- or heterodimers (PDGF-AA, -AB, -BB, -CC, -DD). The four PDGFs are inactive in their monomeric forms. The PDGFs bind to the protein tyrosine kinase receptors PDGF receptor-α and -β. These two receptor isoforms dimerize upon binding the PDGF dimer, leading to three possible receptor combinations, namely -αα, -ββ and -αβ. PDGF-CC specifically interacts with PDGFR-αα and -αβ, but not with –ββ, and thereby resembles PDGF-AB. PDGF-DD binds to PDGFR-ββ with high affinity and to PDGFR-αβ to a much lower extent and is regarded as PDGFR-ββ specific. PDGF-AA binds only to PDGFR-αα, while PDGF-BB is the only PDGF that can bind all three receptor combinations with high affinity.
Concentration
N/A
Molecular weight
This protein contains C-terminal polyhistidine tag and has a calculated MW of 57.1 kDa. As a result of glycosylation, DTT-reduced protein migrates as 85-95 kDa polypeptide in SDS-PAGE.
Synonym
PDGFRB, PDGFR-B, CD140B, CD-140B, JTK12, JTK-12, PDGF-R-beta, PDGFR, PDGFR1, PDGFR-1, PDGFR-beta, PDGFR-β, PDGF-R-β
Other name
PDGFRB, PDGFR-B, CD140B, CD-140B, JTK12, JTK-12, PDGF-R-beta, PDGFR, PDGFR1, PDGFR-1, PDGFR-beta, PDGFR-β, PDGF-R-β
NCBI gene number
5159
NCBI gene
PDGFRB
Gene source
Human
NCBI number
P09619
Recombinant
Yes
Source
HEK293 cells
Purification
≥95%
Tested applications
SDS-PAGE, N/A
Tested activity
N/A
Biological activity
Measured by its ability to inhibit the biological activity of PDGFBB using NR6R-3T3 mouse fibroblast cells. The ED50 for this effect is typically 3-8 μg/ml in the presence of 4 ng/ml rhPDGFBB.
Reesults
Measured by its ability to inhibit the biological activity of PDGFBB using NR6R-3T3 mouse fibroblast cells. The ED50 for this effect is typically 3-8 μg/ml in the presence of 4 ng/ml rhPDGFBB.
Binding ability
N/A
Unit
N/A
Storage condition
-20°C
Shipping under
Gel Pack
Physical appearance
Lyophilized
Physical properties
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
How to reconstitute
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants