Human CellExp™ KIM3/HAVCR2, human recombinant

Catalog number
7495-10
Name
Human CellExp™ KIM3/HAVCR2, human recombinant
Size
10 μg
Price
246.00 EUR
Supplier

Details

Target
KIM3/HAVCR2
Background
Hepatitis A virus cellular receptor 2 also known as HAVCR2, FLJ14428, KIM3, TIM3, TIMD3, is a member of the TIM family of immune regulating molecules with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk. CD4-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells and their associated cytokines are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. The 2 types of cells also cross-regulate the functions of the other. HAVCR2 is a Th1-specific cell surface protein that regulates macrophage activation and enhances the severity of experimental autoimmune encephalomyelitis in mice. HAVCR2 regulates macrophage activation. Inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance. May be also involved in T-cell homing. Dysregulation of the HAVCR2-galectin-9 pathway could underlie chronic autoimmune disease states in human, such as multiple sclerosis.
Concentration
N/A
Molecular weight
This protein is fused with the Fc region of human IgG1 at the C-terminus and has a calculated MW of 42 kDa after removal of signal peptide. In DTT-reduced SDS-PAGE, rhKIM3-Fc protein migrates as 45 kDa poly peptide due to glycosylation.
Synonym
HAVCR2, TIM3, TIMD3, FLJ14428, KIM3
Other name
HAVCR2, TIM3, TIMD3, FLJ14428, KIM3
NCBI gene number
84868
NCBI gene
HAVCR2
Gene source
Human
NCBI number
Q8TDQ0
Recombinant
Yes
Source
HEK293 cells
Purification
≥97%
Tested applications
SDS-PAGE, N/A
Tested activity
N/A
Biological activity
Measured by its binding ability in a functional ELISA. Immobilized rh-Galectin-9 at 500 ng/ml can bind rhKIM3-Fc with an apparent KD
Reesults
Measured by its binding ability in a functional ELISA. Immobilized rh-Galectin-9 at 500 ng/ml can bind rhKIM3-Fc with an apparent KD
Binding ability
N/A
Unit
N/A
Storage condition
-20°C
Shipping under
Gel Pack
Physical appearance
Lyophilized
Physical properties
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM glycine, pH 7.0. Normally Mannitol or Trehalose is added as protectants before lyophilization.
How to reconstitute
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants