Human CellExp™ CSF1R / CD115 / CD6, human recombinant

Catalog number
7505-100
Name
Human CellExp™ CSF1R / CD115 / CD6, human recombinant
Size
100 μg
Price
719.00 EUR
Supplier

Details

Target
CSF1R / CD115 / CD6
Background
Colony stimulating factor 1 receptor (CSF1R), also known as macrophage colony-stimulating factor receptor (M-CSFR), CD115 Cluster of Differentiation 115 (CD115), C-FMS, CSFR, FIM2, FMS, and is a member of the type III subfamily of receptor tyrosine kinases (RTKs). CSF1R is a receptor for a cytokine called colony stimulating factor 1, The protein encoded by the CSFR1 gene is the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. This receptor mediates most, if not all, of the biological effects of this cytokine. Ligand binding activates CSFR1 through a process of oligomerization and transphosphorylation. Mutations in CSF1R are associated with chronic myelomonocytic leukemia and type M4 acute myeloblastic leukemia. Increased levels of CSF1R1 are found in microglia in Alzheimer's disease and after brain injuries. The increased receptor expression causes microglia to become more active. Both CSF1R, and its ligand colony stimulating factor 1 play an important role in the development of the mammary gland and may be involved in the process of mammary gland carcinogenesis.
Concentration
N/A
Molecular weight
This protein rhCSF1R is fused with a C-terminal 6×his tag and has a calculated MW of 55.1 kDa. The predicted N-terminus is Ile 20. DTT-reduced protein migrates as 80-95 kDa polypeptide in SDS-PAGE.
Synonym
CD6, CD-6, Cluster of Differentiation 6, FLJ44171, TP120
Other name
CD6, CD-6, Cluster of Differentiation 6, FLJ44171, TP120
NCBI gene number
1436
NCBI gene
CSF1R
Gene source
Human
NCBI number
P07333
Recombinant
Yes
Source
HEK293 cells
Purification
≥98%
Tested applications
SDS-PAGE, N/A
Tested activity
N/A
Biological activity
Measured by its ability to inhibit the MCSF-induced proliferation of NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is typically 1.5-8.7 pg/ml in the presence of 1 ng/ml of rhMCSF.
Reesults
The ED50 for this effect is typically 1.5-8.7 pg/ml in the presence of 1 ng/ml of rhMCSF.
Binding ability
N/A
Unit
N/A
Storage condition
-20°C
Shipping under
Gel Pack
Physical appearance
Lyophilized
Physical properties
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
How to reconstitute
Centrifuge the vial prior to opening. Reconstitute in PBS, pH 7.4. Do not vortex.
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants