Human CellExp™ Influenza A virus / Neuraminidase (NA)

Catalog number
7508-20
Name
Human CellExp™ Influenza A virus / Neuraminidase (NA)
Size
20 μg
Price
300.00 EUR
Supplier

Details

Target
Influenza A virus / Neuraminidase (NA)
Background
Neuraminidase (NA) and hemagglutinin (HA) are major membrane glycoproteins found on the surface of influenza virus. Hemagglutinin binds to the sialic acid-containing receptors on the surface of host cells during initial infection and at the end of an infectious cycle. Neuraminidase, on the other hand, cleaves the HA-sialic acid bondage from the newly formed virions and the host cell receptors during budding. Neuraminidase thus is described as a receptor-destroying enzyme which facilitates virus release and efficient spread of the progeny virus from cell to cell.
Concentration
N/A
Molecular weight
Influenza A virus (A/Thailand/1(KAN-1)/2004 (H5N1)) Neuraminidase (NA) is fused with a polyhistidine tag at the N-terminus, and has a calculated MW of 46.1 kDa. The predicted N-terminus is His 36. DTT-reduced Protein migrates as 48 kDa in SDS-PAGE
Synonym
NA, Neuraminidase
Other name
NA, Neuraminidase
NCBI gene number
N/A
NCBI gene
neuraminidase/NA
Gene source
Influenza A Virus
NCBI number
Q76UU8
Recombinant
Yes
Source
HEK293 cells
Purification
≥92%
Tested applications
SDS-PAGE, N/A
Tested activity
N/A
Biological activity
Measured by its ability to cleave a fluorogenic substrate, 2'-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic acid. One unit is defined as the amount of enzyme required to cleave 1 nmole of 2'-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic acid per minute at pH 7.5 at 37°C
Reesults
Measured by its ability to cleave a fluorogenic substrate, 2'-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic acid. One unit is defined as the amount of enzyme required to cleave 1 nmole of 2'-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic acid per minute at pH 7.5 at 37°C
Binding ability
N/A
Unit
One unit is defined as the amount of enzyme required to cleave 1 nmole of 2'-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic acid per minute at pH 7.5 at 37°C
Storage condition
-20°C
Shipping under
Gel Pack
Physical appearance
Lyophilized
Physical properties
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
How to reconstitute
Centrifuge the vial prior to opening. Reconstitute in PBS, pH 7.4. Do not vortex.
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Virus
influenza
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Description
Influenza A and B H1N1 H3N2 Hemagglutinin-nucleoprotein recombinant proteins, peptides and antibodies detect a virus commonly known as "the flu". Influenza is an infectious disease caused by an influenza virus. Symptoms can be mild to severe. The most common symptoms include a high fever, runny nose, sore throat, muscle pains, headache, coughing, and feeling tired. These symptoms typically begin two days after exposure to the virus and most last less than a week. The cough, however, may last for more than two weeks. In children, there may be nausea and vomiting, but these are not common in adults.